LOCUS       sequence-162244-        6558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ERBB2_p.S418T
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3958..4057)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4074..4093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4075..4093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4098..4114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4098..4114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4545..5336
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5429..6017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5918..5937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6171..6188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6347..6374)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6466..6552)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgacaact ttgtacaaaa
      181 aagttggcac catggagctg gcggccttgt gccgctgggg gctcctcctc gccctcttgc
      241 cccccggagc cgcgagcacc caagtgtgca ccggcacaga catgaagctg cggctccctg
      301 ccagtcccga gacccacctg gacatgctcc gccacctcta ccagggctgc caggtggtgc
      361 agggaaacct ggaactcacc tacctgccca ccaatgccag cctgtccttc ctgcaggata
      421 tccaggaggt gcagggctac gtgctcatcg ctcacaacca agtgaggcag gtcccactgc
      481 agaggctgcg gattgtgcga ggcacccagc tctttgagga caactatgcc ctggccgtgc
      541 tagacaatgg agacccgctg aacaatacca cccctgtcac aggggcctcc ccaggaggcc
      601 tgcgggagct gcagcttcga agcctcacag agatcttgaa aggaggggtc ttgatccagc
      661 ggaaccccca gctctgctac caggacacga ttttgtggaa ggacatcttc cacaagaaca
      721 accagctggc tctcacactg atagacacca accgctctcg ggcctgccac ccctgttctc
      781 cgatgtgtaa gggctcccgc tgctggggag agagttctga ggattgtcag agcctgacgc
      841 gcactgtctg tgccggtggc tgtgcccgct gcaaggggcc actgcccact gactgctgcc
      901 atgagcagtg tgctgccggc tgcacgggcc ccaagcactc tgactgcctg gcctgcctcc
      961 acttcaacca cagtggcatc tgtgagctgc actgcccagc cctggtcacc tacaacacag
     1021 acacgtttga gtccatgccc aatcccgagg gccggtatac attcggcgcc agctgtgtga
     1081 ctgcctgtcc ctacaactac ctttctacgg acgtgggatc ctgcaccctc gtctgccccc
     1141 tgcacaacca agaggtgaca gcagaggatg gaacacagcg gtgtgagaag tgcagcaagc
     1201 cctgtgcccg agtgtgctat ggtctgggca tggagcactt gcgagaggtg agggcagtta
     1261 ccagtgccaa tatccaggag tttgctggct gcaagaagat ctttgggagc ctggcatttc
     1321 tgccggagag ctttgatggg gacccagcct ccaacactgc cccgctccag ccagagcagc
     1381 tccaagtgtt tgagactctg gaagagatca caggttacct atacatctca gcatggccgg
     1441 acaccctgcc tgacctcagc gtcttccaga acctgcaagt aatccgggga cgaattctgc
     1501 acaatggcgc ctactcgctg accctgcaag ggctgggcat cagctggctg gggctgcgct
     1561 cactgaggga actgggcagt ggactggccc tcatccacca taacacccac ctctgcttcg
     1621 tgcacacggt gccctgggac cagctctttc ggaacccgca ccaagctctg ctccacactg
     1681 ccaaccggcc agaggacgag tgtgtgggcg agggcctggc ctgccaccag ctgtgcgccc
     1741 gagggcactg ctggggtcca gggcccaccc agtgtgtcaa ctgcagccag ttccttcggg
     1801 gccaggagtg cgtggaggaa tgccgagtac tgcaggggct ccccagggag tatgtgaatg
     1861 ccaggcactg tttgccgtgc caccctgagt gtcagcccca gaatggctca gtgacctgtt
     1921 ttggaccgga ggctgaccag tgtgtggcct gtgcccacta taaggaccct cccttctgcg
     1981 tggcccgctg ccccagcggt gtgaaacctg acctctccta catgcccatc tggaagtttc
     2041 cagatgagga gggcgcatgc cagccttgcc ccatcaactg cacccactcc tgtgtggacc
     2101 tggatgacaa gggctgcccc gccgagcaga gagccagccc tctgacgtcc atcatctctg
     2161 cggtggttgg cattctgctg gtcgtggtct tgggggtggt ctttgggatc ctcatcaagc
     2221 gacggcagca gaagatccgg aagtacacga tgcggagact gctgcaggaa acggagctgg
     2281 tggagccgct gacacctagc ggagcgatgc ccaaccaggc gcagatgcgg atcctgaaag
     2341 agacggagct gaggaaggtg aaggtgcttg gatctggcgc ttttggcaca gtctacaagg
     2401 gcatctggat ccctgatggg gagaatgtga aaattccagt ggccatcaaa gtgttgaggg
     2461 aaaacacatc ccccaaagcc aacaaagaaa tcttagacga agcatacgtg atggctggtg
     2521 tgggctcccc atatgtctcc cgccttctgg gcatctgcct gacatccacg gtgcagctgg
     2581 tgacacagct tatgccctat ggctgcctct tagaccatgt ccgggaaaac cgcggacgcc
     2641 tgggctccca ggacctgctg aactggtgta tgcagattgc caaggggatg agctacctgg
     2701 aggatgtgcg gctcgtacac agggacttgg ccgctcggaa cgtgctggtc aagagtccca
     2761 accatgtcaa aattacagac ttcgggctgg ctcggctgct ggacattgac gagacagagt
     2821 accatgcaga tgggggcaag gtgcccatca agtggatggc gctggagtcc attctccgcc
     2881 ggcggttcac ccaccagagt gatgtgtgga gttatggtgt gactgtgtgg gagctgatga
     2941 cttttggggc caaaccttac gatgggatcc cagcccggga gatccctgac ctgctggaaa
     3001 agggggagcg gctgccccag ccccccatct gcaccattga tgtctacatg atcatggtca
     3061 aatgttggat gattgactct gaatgtcggc caagattccg ggagttggtg tctgaattct
     3121 cccgcatggc cagggacccc cagcgctttg tggtcatcca gaatgaggac ttgggcccag
     3181 ccagtccctt ggacagcacc ttctaccgct cactgctgga ggacgatgac atgggggacc
     3241 tggtggatgc tgaggagtat ctggtacccc agcagggctt cttctgtcca gaccctgccc
     3301 cgggcgctgg gggcatggtc caccacaggc accgcagctc atctaccagg agtggcggtg
     3361 gggacctgac actagggctg gagccctctg aagaggaggc ccccaggtct ccactggcac
     3421 cctccgaagg ggctggctcc gatgtatttg atggtgacct gggaatgggg gcagccaagg
     3481 ggctgcaaag cctccccaca catgacccca gccctctaca gcggtacagt gaggacccca
     3541 cagtacccct gccctctgag actgatggct acgttgcccc cctgacctgc agcccccagc
     3601 ctgaatatgt gaaccagcca gatgttcggc cccagccccc ttcgccccga gagggccctc
     3661 tgcctgctgc ccgacctgct ggtgccactc tggaaaggcc caagactctc tccccaggga
     3721 agaatggggt cgtcaaagac gtttttgcct ttgggggtgc cgtggagaac cccgagtact
     3781 tgacacccca gggaggagct gcccctcagc cccaccctcc tcctgccttc agcccagcct
     3841 tcgacaacct ctattactgg gaccaggacc caccagagcg gggggctcca cccagcacct
     3901 tcaaagggac acctacggca gagaacccag agtacctggg tctggacgtg ccagtgtgac
     3961 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     4021 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     4081 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     4141 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     4201 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     4261 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     4321 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     4381 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     4441 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     4501 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     4561 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     4621 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     4681 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     4741 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     4801 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     4861 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     4921 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     4981 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     5041 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     5101 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     5161 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     5221 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     5281 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     5341 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     5401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6301 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     6361 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     6421 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     6481 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     6541 tcgttttatt tgatgcct
//
