LOCUS       sequence-162248-        4917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FBXW7 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_FBXW7_p.D211N
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2317..2416)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2433..2452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2434..2452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2457..2473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2457..2473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2904..3695
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3788..4376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4277..4296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4530..4547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4706..4733)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4825..4911)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgaatcag gaactgctct ctgtgggcag caaaagacga cgaactggag
      241 gctctctgag aggtaaccct tcctcaagcc aggtagatga agaacagatg aatcgtgtgg
      301 tagaggagga acagcaacag caactcagac aacaagagga ggagcacact gcaaggaatg
      361 gtgaagttgt tggagtagaa cctagacctg gaggccaaaa tgattcccag caaggacagt
      421 tggaagaaaa caataataga tttatttcgg tagatgagga ctcctcagga aaccaagaag
      481 aacaagagga agatgaagaa catgctggtg aacaagatga ggaggatgag gaggaggagg
      541 agatggacca ggagagtgac gattttgatc agtctgatga tagtagcaga gaagatgaac
      601 atacacatac taacagtgtc acgaactcca gtagtattgt ggacctgccc gttcaccaac
      661 tctcctcccc attctataca aaaacaacaa aaatgaaaag aaagttggac catggttctg
      721 aggtccgctc tttttctttg ggaaagaaac catgcaaagt ctcagaatat acaagtacca
      781 ctgggcttgt accatgttca gcaacaccaa caacttttgg gaacctcaga gcagccaatg
      841 gccaagggca acaacgacgc cgaattacat ctgtccagcc acctacaggc ctccaggaat
      901 ggctaaaaat gtttcagagc tggagtggac cagagaaatt gcttgcttta gatgaactca
      961 ttgatagttg tgaaccaaca caagtaaaac atatgatgca agtgatagaa ccccagtttc
     1021 aacgagactt catttcattg ctccctaaag agttggcact ctatgtgctt tcattcctgg
     1081 aacccaaaga cctgctacaa gcagctcaga catgtcgcta ctggagaatt ttggctgaag
     1141 acaaccttct ctggagagag aaatgcaaag aagaggggat tgatgaacca ttgcacatca
     1201 agagaagaaa agtaataaaa ccaggtttca tacacagtcc atggaaaagt gcatacatca
     1261 gacagcacag aattgatact aactggaggc gaggagaact caaatctcct aaggtgctga
     1321 aaggacatga tgatcatgtg atcacatgct tacagttttg tggtaaccga atagttagtg
     1381 gttctgatga caacacttta aaagtttggt cagcagtcac aggcaaatgt ctgagaacat
     1441 tagtgggaca tacaggtgga gtatggtcat cacaaatgag agacaacatc atcattagtg
     1501 gatctacaga tcggacactc aaagtgtgga atgcagagac tggagaatgt atacacacct
     1561 tatatgggca tacttccact gtgcgttgta tgcatcttca tgaaaaaaga gttgttagcg
     1621 gttctcgaga tgccactctt agggtttggg atattgagac aggccagtgt ttacatgttt
     1681 tgatgggtca tgttgcagca gtccgctgtg ttcaatatga tggcaggagg gttgttagtg
     1741 gagcatatga ttttatggta aaggtgtggg atccagagac tgaaacctgt ctacacacgt
     1801 tgcaggggca tactaataga gtctattcat tacagtttga tggtatccat gtggtgagtg
     1861 gatctcttga tacatcaatc cgtgtttggg atgtggagac agggaattgc attcacacgt
     1921 taacagggca ccagtcgtta acaagtggaa tggaactcaa agacaatatt cttgtctctg
     1981 ggaatgcaga ttctacagtt aaaatctggg atatcaaaac aggacagtgt ttacaaacat
     2041 tgcaaggtcc caacaagcat cagagtgctg tgacctgttt acagttcaac aagaactttg
     2101 taattaccag ctcagatgat ggaactgtaa aactatggga cttgaaaacg ggtgaattta
     2161 ttcgaaacct agtcacattg gagagtgggg ggagtggggg agttgtgtgg cggatcagag
     2221 cctcaaacac aaagctggtg tgtgcagttg ggagtcggaa tgggactgaa gaaaccaagc
     2281 tgctggtgct ggactttgat gtggacatga agtgattgcc aactttcttg tacaaagttg
     2341 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2401 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2461 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2521 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2581 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2641 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2701 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2761 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2821 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2881 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2941 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3001 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3061 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3121 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3181 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3241 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3301 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3361 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3421 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3481 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3541 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3601 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3661 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3721 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4561 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4621 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4681 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4741 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4801 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4861 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
