LOCUS       sequence-162249-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP11
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(289..307)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(290..307)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(327..344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    333..557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      603..961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(690..709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     900..921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attctgcaga tctccaacct cacaccacat tacactgggg tgatataata
      181 tgatgccggg cgggggccgt aacgtagcac tacatgaccc gatacgctag gtgggattag
      241 gatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca
      301 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
      541 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
      601 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      961 ataaggga
//
