LOCUS       sequence-162252-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP12
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(197..215)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(198..215)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(235..252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    241..465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      511..939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(598..617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     808..829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(948..968)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgggatcccg taccatacta taccgtggcg
      121 cggcgtgaca tcgcaccgca tggcacgtca ggctgagatt ccaggtcgga ctccaacctc
      181 acctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
      241 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      301 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      361 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      421 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      481 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      541 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      601 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      661 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      721 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      781 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      841 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      901 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
      961 gttagggtgt ggaaagtccc caggctcccc a
//
