LOCUS       sequence-162258-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a synthetic insert to serve as internal control DNA 
            template for a Salmonella Real-Time PCR assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     FDA ORA lab InC plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCR2.1-InC
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(385..813)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(495..516)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     707..726
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     939..961
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     953..970
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     954..970
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        977..995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
       61 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
      121 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
      181 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
      241 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
      301 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
      361 cccttgcgcc ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      421 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      481 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      541 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      601 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      661 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      721 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      781 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
      841 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      901 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      961 gacggccagt gaattgtaat acgactcact atagg
//
