LOCUS       sequence-162267-        5214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FGFR3, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_FGFR3_p.R399H
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2614..2713)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2730..2749)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2731..2749)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2754..2770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2754..2770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3201..3992
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4085..4673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4574..4593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4827..4844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5003..5030)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5122..5208)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgggcgcc cctgcctgcg ccctcgcgct ctgcgtggcc gtggccatcg
      241 tggccggcgc ctcctcggag tccttgggga cggagcagcg cgtcgtgggg cgagcggcag
      301 aagtcccggg cccagagccc ggccagcagg agcagttggt cttcggcagc ggggatgctg
      361 tggagctgag ctgtcccccg cccgggggtg gtcccatggg gcccactgtc tgggtcaagg
      421 atggcacagg gctggtgccc tcggagcgtg tcctggtggg gccccagcgg ctgcaggtgc
      481 tgaatgcctc ccacgaggac tccggggcct acagctgccg gcagcggctc acgcagcgcg
      541 tactgtgcca cttcagtgtg cgggtgacag acgctccatc ctcgggagat gacgaagacg
      601 gggaggacga ggctgaggac acaggtgtgg acacaggggc cccttactgg acacggcccg
      661 agcggatgga caagaagctg ctggccgtgc cggccgccaa caccgtccgc ttccgctgcc
      721 cagccgctgg caaccccact ccctccatct cctggctgaa gaacggcagg gagttccgcg
      781 gcgagcaccg cattggaggc atcaagctgc ggcatcagca gtggagcctg gtcatggaaa
      841 gcgtggtgcc ctcggaccgc ggcaactaca cctgcgtcgt ggagaacaag tttggcagca
      901 tccggcagac gtacacgctg gacgtgctgg agcgctcccc gcaccggccc atcctgcagg
      961 cggggctgcc ggccaaccag acggcggtgc tgggcagcga cgtggagttc cactgcaagg
     1021 tgtacagtga cgcacagccc cacatccagt ggctcaagca cgtggaggtg aacggcagca
     1081 aggtgggccc ggacggcaca ccctacgtta ccgtgctcaa gacggcgggc gctaacacca
     1141 ccgacaagga gctagaggtt ctctccttgc acaacgtcac ctttgaggac gccggggagt
     1201 acacctgcct ggcgggcaat tctattgggt tttctcatca ctctgcgtgg ctggtggtgc
     1261 tgccagccga ggaggagctg gtggaggctg acgaggcggg cagtgtgtat gcaggcatcc
     1321 tcagctacgg ggtgggcttc ttcctgttca tcctggtggt ggcggctgtg acgctctgcc
     1381 gcctgcacag cccccccaag aaaggcctgg gctcccccac cgtgcacaag atctcccgct
     1441 tcccgctcaa gcgacaggtg tccctggagt ccaacgcgtc catgagctcc aacacaccac
     1501 tggtgcgcat cgcaaggctg tcctcagggg agggccccac gctggccaat gtctccgagc
     1561 tcgagctgcc tgccgacccc aaatgggagc tgtctcgggc ccggctgacc ctgggcaagc
     1621 cccttgggga gggctgcttc ggccaggtgg tcatggcgga ggccatcggc attgacaagg
     1681 accgggccgc caagcctgtc accgtagccg tgaagatgct gaaagacgat gccactgaca
     1741 aggacctgtc ggacctggtg tctgagatgg agatgatgaa gatgatcggg aaacacaaaa
     1801 acatcatcaa cctgctgggc gcctgcacgc agggcgggcc cctgtacgtg ctggtggagt
     1861 acgcggccaa gggtaacctg cgggagtttc tgcgggcgcg gcggcccccg ggcctggact
     1921 actccttcga cacctgcaag ccgcccgagg agcagctcac cttcaaggac ctggtgtcct
     1981 gtgcctacca ggtggcccgg ggcatggagt acttggcctc ccagaagtgc atccacaggg
     2041 acctggctgc ccgcaatgtg ctggtgaccg aggacaacgt gatgaagatc gcagacttcg
     2101 ggctggcccg ggacgtgcac aacctcgact actacaagaa gacaaccaac ggccggctgc
     2161 ccgtgaagtg gatggcgcct gaggccttgt ttgaccgagt ctacactcac cagagtgacg
     2221 tctggtcctt tggggtcctg ctctgggaga tcttcacgct ggggggctcc ccgtaccccg
     2281 gcatccctgt ggaggagctc ttcaagctgc tgaaggaggg ccaccgcatg gacaagcccg
     2341 ccaactgcac acacgacctg tacatgatca tgcgggagtg ctggcatgcc gcgccctccc
     2401 agaggcccac cttcaagcag ctggtggagg acctggaccg tgtccttacc gtgacgtcca
     2461 ccgacgagta cctggacctg tcggcgcctt tcgagcagta ctccccgggt ggccaggaca
     2521 cccccagctc cagctcctca ggggacgact ccgtgtttgc ccacgacctg ctgcccccgg
     2581 ccccacccag cagtgggggc tcgcggacgt gattgccaac tttcttgtac aaagttggca
     2641 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2701 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2761 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2821 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2881 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2941 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     3001 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     3061 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3121 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3181 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3241 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3301 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3361 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3421 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3481 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3541 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3601 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3661 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3721 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3781 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3841 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3901 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3961 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     4021 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4741 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4801 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4981 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     5041 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     5101 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     5161 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
