LOCUS       sequence-162270-       11525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 11525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSGK[MCS]
FEATURES             Location/Qualifiers
     source          1..11525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6158..6946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             7203..7919
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7233..7261)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7856..7876
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(8064..8082)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8065..8082)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8100..8116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8100..8116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8113..8135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8124..8140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8148..8154,8155..8172,8173..8178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8193..8214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8328..8349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8578..9393)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8578..9393)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9301..9320
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9385..9404
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9515..10103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10004..10023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10257..10274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10289..10428
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10354..10373)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10514..10536
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10530..10721)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     11471..11493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11485..11502
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11486..11502
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(11509..11525,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(11509..11525,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cctaaggaca tagtcgctag caagcggccg catttaaatc
       61 cgcggcctag gtcccgggta ccctcagcat acatcttttt aagtcgggca ttctccgatt
      121 ctaatgcctt tagcttggta atcatcggta aatccatacc tccatacctt gatctctata
      181 tagacataat ttggctgtca ctatgctttg atatagtcat tttacagaat ctcctttttt
      241 gttattagaa aattctactc ttcttgtcga ttttgtttta gggaggatta ccttctctta
      301 tgtttttgat cctgtgtttt cacttacttt gactataatt ttttaactaa tatccatact
      361 ccactctctt aacccctgca ctagagccaa gcctctgtat aacatgagag taattattac
      421 gcacccaatc cgcaataaat cttgctggca ttgtaaactt tagtgtagaa gtatcattgc
      481 actccttcac tactgctttt tcaaaccaag ctgtatacat agcttctcct aactcttcaa
      541 ttaatccttg tctgatatga tgccatgctg tatttgcttc gatcgtatct aatcccccac
      601 ttttcgctaa aatacctgca tgtgtaccat cagacacaac atcagatgtt ataattttag
      661 gatatagcat taccttttct tcatctctcc gctttctttc attgcttgta tcctccacct
      721 cttctacctt ctgcacataa taaccgttca ttccatatac tgtttctagc tgctcactta
      781 agatctctat ttgtcgctca cttaaagcta ggttacttgg aattaataca gttataaagc
      841 tgttgttggc acttatctta aactctactt gagttagtat tgaatacgct atggaagtgc
      901 taaatcttcc tgctatcttc ttctttacct gcatttcttt actggtatca aatgaattct
      961 ctatttggct taaatattgc tcatgttcta acatgttctg ctcttcagca cctatattac
     1021 aactgaatct aaatgttgta tgattaacaa gtggtccttg atgtttttcg ctcgcaagca
     1081 ctttcttcat atagcttaag aagataaact tattcttaaa tttcttttca ggatatttga
     1141 tatatagctt caatagtaac tggtttacaa agttagtgct aaattccctg tttgctttga
     1201 agttaagtat atctacatct ttttctgata gtggatagaa atgccttaat tctttatttt
     1261 cagatatatc tttgatggtt cttgcaacaa tctcggggtg tatattagca caaggtagct
     1321 taagactagc tgtttcatgg tattcttcct tagaaacaaa acaaccagct tcagaatcat
     1381 ttaaaaaaga agaattgtta taaaattcag atggaaacga gctttgctcg tttcccaaaa
     1441 actctttttt gaaattttct tcagatttat atatgtttga agaatcattt atagataaat
     1501 tcttaagatt tacatctata taatctatat ttttaataat actattcttt gatacaaagt
     1561 ctttactata taattgacca gaatcagtgc aaggagggtc atattctgtg catttccatt
     1621 ctttagcgat ttctaaggct ttattgttat tatactcaat tactatttgt tgcttttgct
     1681 ctttctgttt tttctgatca ttgttattta tgagagatat attcagaaga gatatttgcc
     1741 ataaagattt atcagccctg ctattataat catcattgtt tctgataact aacttatctc
     1801 gcttaataag attattatct tctaaatcct ttaatgctct agaaatagaa tttctatgta
     1861 atttaagttc tttctgtaat tcttgataag aagtgacaat agagtgcaga ttattagtta
     1921 tagagtgatc atggtcataa gcatgatcat aaaaatcttt gtaatcattc ttattgctgt
     1981 tactggcatc aatataagtc tctgtagtga tcctgttgtc tttgtcctta ctgtccgtgt
     2041 ctctgttacc tttatcatta ttactagata atgaagattt ataaatcttt gtaaaaagat
     2101 agagtgttat agtctttgct gttgcagaaa tgctgcaatt agaatttaat tgctttaaga
     2161 actcataagg tactcttata aacatcttag ttttttcaag atgttgcctt tttccttcta
     2221 tggttggcac aaagatatta tctatagaat ctaacttatc tgacttagag tgacgtgttt
     2281 ttgtattatc gatatcacct agatcaaacc tatcaggttc atatttaaga tcattaaaaa
     2341 ccttatcagg tattgttgta gtaataatat tacgtttatt ctgaccttga tcgttcttat
     2401 ctctagtaat aatgaaatgt cctttagctt ccagggattt ctgaagcaca aatacttcag
     2461 atttagagca agatagtctt tctgcccagc ttttagcagg tagagatata gatctattat
     2521 ggcccttaga gaagttaata caagaaagcc catcagctaa gatgtagtac attttctcaa
     2581 gagtagataa ctctttgctt gataatatat gagataatac gcacccagtt attgggacgt
     2641 atgattgatt ctttgtggta tcatagtact gatgtatacc tacattatca ctgggcgtag
     2701 caagcgggta aaatgattgc attttatgct ccttttatgg agctaagtac ctgaaaccct
     2761 tgaccgctac gtagaaatag tatatattta ttatatgtaa aaatcattct gcccgtgttt
     2821 atttttgcat atcatatcaa tcaaggtgtt gagttttagc tattttgagc acttagcacc
     2881 tgtagtttat tgtttcacta tcgtatctat tttatttaat ttatacttct cgagtttatc
     2941 atttgcagca tctataaacc actttctgcg cgtgataaag ttttcagcac aaattttatc
     3001 aatcttttct ataatatgct ctggaatatc taaagatatc ctttttacgt ttattttctt
     3061 cagcataaag ccattgagat aaatgaatta taatcatagt taaacatatt ttgtacacat
     3121 gtcaataaaa ataattattt cacacataaa aaataattat tttaaaatca gagcacataa
     3181 catgtatatg ttaacatata tgtatgtttt tacaatctaa tataaacata ttgacatatg
     3241 tatgtttata tatttatatt atcgtataaa aaataaataa tgttattatg attttgtctt
     3301 tcttgaatca aaaaggaggt gtaggtaaga ctacactttc aattaatgtt gcaacatgtc
     3361 tatcactaaa aaagcaaaaa gtgcttttaa tagatgcgga tccacaaaat agttctctag
     3421 attgggttgc aactcgtaga aaagaaaatt tattcactgt agttgggctc acaaaaccaa
     3481 taatacataa agaggtatca aaattggtta agaattatga tcatataatt attgatggac
     3541 caccaaggat atatgatgtt gctaagtctg caatagttac tagtgacctg gtagtaatgc
     3601 cagtgcaacc gtcaccttat gatatatggg cagctaatga agtggttagc cttataaaag
     3661 aagtatctca accattagaa ggaataaaaa atataaagtc cgccttttta attaatagga
     3721 aaatatccaa tactgctatt ggtcgagatg tggaacaagc gttagatcat tacggtatgg
     3781 atattttaca aacacatatt tgtcagagag tggcatatgc agaaacagca gctattggtt
     3841 ccacagttat ggaaaagcat aatagagaca atctagccgt tagagaaata acagatttaa
     3901 caaatgaaat tttagaaaaa tataactaat tgaaaaggtc atgacaagaa aaagaattgc
     3961 gctccccaaa aaccggtaca aaaaaatact actcaattta ttgatgcatg ggtgtcgggt
     4021 aatataaaac atgaacaaca ggatcaaaaa cataagagaa cgactatctt tttaccagaa
     4081 gaattgcata agaaattaaa aatcgaggcg gctaggaggg aggtaacaat gactgaaata
     4141 attatagaag ctgtagaaaa aagtatatag acatatatat gcttatatgt cttaacttaa
     4201 tgtttttgtt tattaaagca aggatgctta atgttaagca ggatcaaata ctattcatca
     4261 cgtttcttga tcagacttat aactaaattt tagtgtagta caaatcatat aaacatatag
     4321 gaatatgtat gtttatattt tggtcctacc aatcatttca tctatagcga tttgaaaata
     4381 atctgccagt tttactataa ctttaatact taaagtccta ttatcactat ttgtattaat
     4441 aaaatgagta atggtactac tacctactct gcaatttttt tccaatatat actctgtaat
     4501 gttaagttgc tgtatttttg ttttaagaaa agagaataag ttattattaa tatcatttaa
     4561 agttaaagat acatataatt gatatttttt tgtatttttt ataaattttg tgcgaccaag
     4621 tacttcatct atatcacatt caaagaatgt tgcaattata agaatagtga tgatattagg
     4681 attaagtaaa attgcgttta ggagtttgct aatagtagat cgcgataaag atgttcctaa
     4741 taccaaatct ttttgttgta ttttataagc tttaattttt tgacttaaaa atttttgtat
     4801 ccttagtgca atatccataa ataactcaaa atatcataca aatagtttct gaatgccaaa
     4861 aactattttc ttgacacatt tcttgacaca ccaaaaatat atttaacaaa gtagaagtat
     4921 gaggtagtgg attgagctga taataaagta caaactatct tataaaaaga aaaaccaata
     4981 aacactcaaa ttttggacaa ggagcgtgtt gactggtgat taaagtcacc caaactgcaa
     5041 gattagttca catatcttat gaccctaatg ttacgagttt tgtagatcaa gcgtgcaatg
     5101 ctgacaatgc agatcatgat aaccaatcct catcaaaatt acctaccgct aaaacaatca
     5161 cagtgctaat cagtttttta aaactcagca aatatgacga tgagtggtta agagtaggga
     5221 agcaactact gctactgcaa agacctacag ataagcgaag atgctatagg tcagttttat
     5281 tatgttttaa gtcagtaatc aaaacccatt ctgataatta cgtagcatat tactttagcg
     5341 gtataacatt atgtttgctt ggaagttatg acgaagcaat aaagatgttt gatatgagtc
     5401 ttcgctataa caaagaaaaa agcactgctc ttgtacatac atttatctga ggtatcaagc
     5461 atcacagagg gcatcagact tgctacagga aataataaca gtggtaatag tgattgatgc
     5521 acccatgcag aaatgttttt tgttaaataa cgagagttta acagtgaatg agggtcctag
     5581 acaagctaga gaggaaaatc attaaaaaac ttcaaagata caaaatagat ggggtaggga
     5641 aaattagtgc gactaatcac aaacaacgtt cacccaaagg taaaatattg gcatcagcag
     5701 aacaagaaaa atggggtaga tattttgaac tagggatgta ttggcacgga agagaagagg
     5761 gaaatcataa agaattaata tatttgagaa ggtgcttgca gctaaatccc gagaatgaac
     5821 cagcaaattg ttatgtgggg atgatactgc aaaagcaagg cagatatcat gagagcttag
     5881 cggcttacag aaaagcaata tcattaagac ctgagctaaa ggaattagac tcgaagaatg
     5941 cccgacttaa aaagatgtat gctgagggga tcctctagag tcgacctgca gctgccgggg
     6001 atcctctaga ggatccgcaa caaggccaac accgctacgg aatgacatat aatctataca
     6061 taaaaatatc atctatgtta ttaattataa tagacatatt aaaaaagttg cgttataaca
     6121 ctttttaagt gatttaattt tgattaaggt tttacatatg agggaagcgg tgatcgccga
     6181 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6241 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6301 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6361 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6421 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6481 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     6541 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     6601 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6661 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6721 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6781 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6841 gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     6901 tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataagtta agcaaccgtt
     6961 ctataatcat aaaaaaagcc tatatagttt gactatatag gcttttttgc tttataatgt
     7021 agttttgaga agcgtcattg cgaggatcct ctagaggatc cgcaacaagg ccaacaccgc
     7081 tacggaatga catataatct atacataaaa atatcatcta tgttattaat tataatagac
     7141 atattaaaaa agttgcgtta taacactttt taagtgattt aattttgatt aaggttttac
     7201 atatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     7261 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     7321 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     7381 ttgtcactac tttctcttat ggtgttcaat gcttttcccg ttatccggat catatgaaac
     7441 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
     7501 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
     7561 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca
     7621 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
     7681 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag
     7741 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     7801 acctgtcgac acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
     7861 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaataag
     7921 gctaataagc aaccgttcta taatcataaa aaaagcctat atagtttgac tatataggct
     7981 tttttgcttt ataatgtagt tttgagaagc gtcattgcga ggatccccag cttgcatgcc
     8041 tgcaggcatg caagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
     8101 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8161 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8221 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagtgggc catcgccctg
     8281 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8341 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8401 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8461 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8521 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     8581 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     8641 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     8701 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     8761 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     8821 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     8881 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     8941 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     9001 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     9061 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     9121 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     9181 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     9241 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     9301 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     9361 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     9421 agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9661 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10201 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10261 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
    10321 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10381 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
    10441 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
    10501 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
    10561 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
    10621 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
    10681 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
    10741 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
    10801 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
    10861 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
    10921 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
    10981 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
    11041 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
    11101 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
    11161 gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc
    11221 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
    11281 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
    11341 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
    11401 ataagtgcgg cgacgctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    11461 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
    11521 ctata
//
