LOCUS       sequence-162271-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSFA[MCS]
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6158..6946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     7949..7969
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(8157..8175)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8158..8175)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8193..8209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8193..8209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8206..8228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8217..8233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8241..8247,8248..8265,8266..8271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8286..8307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8424..8441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8595..9183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8675..8694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9354..10145,10146..10214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9977..9996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10215..10319)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10387..10405
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10443..10465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10565..10584
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10775..10797
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10789..10806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10790..10806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10813..10829,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10813..10829,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cctaaggaca tagtcgctag caagcggccg catttaaatc
       61 cgcggcctag gtcccgggta ccctcagcat acatcttttt aagtcgggca ttctccgatt
      121 ctaatgcctt tagcttggta atcatcggta aatccatacc tccatacctt gatctctata
      181 tagacataat ttggctgtca ctatgctttg atatagtcat tttacagaat ctcctttttt
      241 gttattagaa aattctactc ttcttgtcga ttttgtttta gggaggatta ccttctctta
      301 tgtttttgat cctgtgtttt cacttacttt gactataatt ttttaactaa tatccatact
      361 ccactctctt aacccctgca ctagagccaa gcctctgtat aacatgagag taattattac
      421 gcacccaatc cgcaataaat cttgctggca ttgtaaactt tagtgtagaa gtatcattgc
      481 actccttcac tactgctttt tcaaaccaag ctgtatacat agcttctcct aactcttcaa
      541 ttaatccttg tctgatatga tgccatgctg tatttgcttc gatcgtatct aatcccccac
      601 ttttcgctaa aatacctgca tgtgtaccat cagacacaac atcagatgtt ataattttag
      661 gatatagcat taccttttct tcatctctcc gctttctttc attgcttgta tcctccacct
      721 cttctacctt ctgcacataa taaccgttca ttccatatac tgtttctagc tgctcactta
      781 agatctctat ttgtcgctca cttaaagcta ggttacttgg aattaataca gttataaagc
      841 tgttgttggc acttatctta aactctactt gagttagtat tgaatacgct atggaagtgc
      901 taaatcttcc tgctatcttc ttctttacct gcatttcttt actggtatca aatgaattct
      961 ctatttggct taaatattgc tcatgttcta acatgttctg ctcttcagca cctatattac
     1021 aactgaatct aaatgttgta tgattaacaa gtggtccttg atgtttttcg ctcgcaagca
     1081 ctttcttcat atagcttaag aagataaact tattcttaaa tttcttttca ggatatttga
     1141 tatatagctt caatagtaac tggtttacaa agttagtgct aaattccctg tttgctttga
     1201 agttaagtat atctacatct ttttctgata gtggatagaa atgccttaat tctttatttt
     1261 cagatatatc tttgatggtt cttgcaacaa tctcggggtg tatattagca caaggtagct
     1321 taagactagc tgtttcatgg tattcttcct tagaaacaaa acaaccagct tcagaatcat
     1381 ttaaaaaaga agaattgtta taaaattcag atggaaacga gctttgctcg tttcccaaaa
     1441 actctttttt gaaattttct tcagatttat atatgtttga agaatcattt atagataaat
     1501 tcttaagatt tacatctata taatctatat ttttaataat actattcttt gatacaaagt
     1561 ctttactata taattgacca gaatcagtgc aaggagggtc atattctgtg catttccatt
     1621 ctttagcgat ttctaaggct ttattgttat tatactcaat tactatttgt tgcttttgct
     1681 ctttctgttt tttctgatca ttgttattta tgagagatat attcagaaga gatatttgcc
     1741 ataaagattt atcagccctg ctattataat catcattgtt tctgataact aacttatctc
     1801 gcttaataag attattatct tctaaatcct ttaatgctct agaaatagaa tttctatgta
     1861 atttaagttc tttctgtaat tcttgataag aagtgacaat agagtgcaga ttattagtta
     1921 tagagtgatc atggtcataa gcatgatcat aaaaatcttt gtaatcattc ttattgctgt
     1981 tactggcatc aatataagtc tctgtagtga tcctgttgtc tttgtcctta ctgtccgtgt
     2041 ctctgttacc tttatcatta ttactagata atgaagattt ataaatcttt gtaaaaagat
     2101 agagtgttat agtctttgct gttgcagaaa tgctgcaatt agaatttaat tgctttaaga
     2161 actcataagg tactcttata aacatcttag ttttttcaag atgttgcctt tttccttcta
     2221 tggttggcac aaagatatta tctatagaat ctaacttatc tgacttagag tgacgtgttt
     2281 ttgtattatc gatatcacct agatcaaacc tatcaggttc atatttaaga tcattaaaaa
     2341 ccttatcagg tattgttgta gtaataatat tacgtttatt ctgaccttga tcgttcttat
     2401 ctctagtaat aatgaaatgt cctttagctt ccagggattt ctgaagcaca aatacttcag
     2461 atttagagca agatagtctt tctgcccagc ttttagcagg tagagatata gatctattat
     2521 ggcccttaga gaagttaata caagaaagcc catcagctaa gatgtagtac attttctcaa
     2581 gagtagataa ctctttgctt gataatatat gagataatac gcacccagtt attgggacgt
     2641 atgattgatt ctttgtggta tcatagtact gatgtatacc tacattatca ctgggcgtag
     2701 caagcgggta aaatgattgc attttatgct ccttttatgg agctaagtac ctgaaaccct
     2761 tgaccgctac gtagaaatag tatatattta ttatatgtaa aaatcattct gcccgtgttt
     2821 atttttgcat atcatatcaa tcaaggtgtt gagttttagc tattttgagc acttagcacc
     2881 tgtagtttat tgtttcacta tcgtatctat tttatttaat ttatacttct cgagtttatc
     2941 atttgcagca tctataaacc actttctgcg cgtgataaag ttttcagcac aaattttatc
     3001 aatcttttct ataatatgct ctggaatatc taaagatatc ctttttacgt ttattttctt
     3061 cagcataaag ccattgagat aaatgaatta taatcatagt taaacatatt ttgtacacat
     3121 gtcaataaaa ataattattt cacacataaa aaataattat tttaaaatca gagcacataa
     3181 catgtatatg ttaacatata tgtatgtttt tacaatctaa tataaacata ttgacatatg
     3241 tatgtttata tatttatatt atcgtataaa aaataaataa tgttattatg attttgtctt
     3301 tcttgaatca aaaaggaggt gtaggtaaga ctacactttc aattaatgtt gcaacatgtc
     3361 tatcactaaa aaagcaaaaa gtgcttttaa tagatgcgga tccacaaaat agttctctag
     3421 attgggttgc aactcgtaga aaagaaaatt tattcactgt agttgggctc acaaaaccaa
     3481 taatacataa agaggtatca aaattggtta agaattatga tcatataatt attgatggac
     3541 caccaaggat atatgatgtt gctaagtctg caatagttac tagtgacctg gtagtaatgc
     3601 cagtgcaacc gtcaccttat gatatatggg cagctaatga agtggttagc cttataaaag
     3661 aagtatctca accattagaa ggaataaaaa atataaagtc cgccttttta attaatagga
     3721 aaatatccaa tactgctatt ggtcgagatg tggaacaagc gttagatcat tacggtatgg
     3781 atattttaca aacacatatt tgtcagagag tggcatatgc agaaacagca gctattggtt
     3841 ccacagttat ggaaaagcat aatagagaca atctagccgt tagagaaata acagatttaa
     3901 caaatgaaat tttagaaaaa tataactaat tgaaaaggtc atgacaagaa aaagaattgc
     3961 gctccccaaa aaccggtaca aaaaaatact actcaattta ttgatgcatg ggtgtcgggt
     4021 aatataaaac atgaacaaca ggatcaaaaa cataagagaa cgactatctt tttaccagaa
     4081 gaattgcata agaaattaaa aatcgaggcg gctaggaggg aggtaacaat gactgaaata
     4141 attatagaag ctgtagaaaa aagtatatag acatatatat gcttatatgt cttaacttaa
     4201 tgtttttgtt tattaaagca aggatgctta atgttaagca ggatcaaata ctattcatca
     4261 cgtttcttga tcagacttat aactaaattt tagtgtagta caaatcatat aaacatatag
     4321 gaatatgtat gtttatattt tggtcctacc aatcatttca tctatagcga tttgaaaata
     4381 atctgccagt tttactataa ctttaatact taaagtccta ttatcactat ttgtattaat
     4441 aaaatgagta atggtactac tacctactct gcaatttttt tccaatatat actctgtaat
     4501 gttaagttgc tgtatttttg ttttaagaaa agagaataag ttattattaa tatcatttaa
     4561 agttaaagat acatataatt gatatttttt tgtatttttt ataaattttg tgcgaccaag
     4621 tacttcatct atatcacatt caaagaatgt tgcaattata agaatagtga tgatattagg
     4681 attaagtaaa attgcgttta ggagtttgct aatagtagat cgcgataaag atgttcctaa
     4741 taccaaatct ttttgttgta ttttataagc tttaattttt tgacttaaaa atttttgtat
     4801 ccttagtgca atatccataa ataactcaaa atatcataca aatagtttct gaatgccaaa
     4861 aactattttc ttgacacatt tcttgacaca ccaaaaatat atttaacaaa gtagaagtat
     4921 gaggtagtgg attgagctga taataaagta caaactatct tataaaaaga aaaaccaata
     4981 aacactcaaa ttttggacaa ggagcgtgtt gactggtgat taaagtcacc caaactgcaa
     5041 gattagttca catatcttat gaccctaatg ttacgagttt tgtagatcaa gcgtgcaatg
     5101 ctgacaatgc agatcatgat aaccaatcct catcaaaatt acctaccgct aaaacaatca
     5161 cagtgctaat cagtttttta aaactcagca aatatgacga tgagtggtta agagtaggga
     5221 agcaactact gctactgcaa agacctacag ataagcgaag atgctatagg tcagttttat
     5281 tatgttttaa gtcagtaatc aaaacccatt ctgataatta cgtagcatat tactttagcg
     5341 gtataacatt atgtttgctt ggaagttatg acgaagcaat aaagatgttt gatatgagtc
     5401 ttcgctataa caaagaaaaa agcactgctc ttgtacatac atttatctga ggtatcaagc
     5461 atcamagagg gcatcagact tgctacagga aataataaca gtggtaatag tgattgatgc
     5521 acccatgcag aaatgttttt tgttaaataa cgagagttta acagtgaatg agggtcctag
     5581 acaagctaga gaggaaaatc attaaaaaac ttcaaagata caaaatagat ggggtaggga
     5641 aaattagtgc gactaatcac aaacaacgtt cacccaaagg taaaatattg gcatcagcag
     5701 aacaagaaaa atggggtaga tattttgaac tagggatgta ttggcacgga agagaagagg
     5761 gaaatcataa agaattaata tatttgagaa ggtgcttgca gctaaatccc gagaatgaac
     5821 cagcaaattg ttatgtgggg atgatactgc aaaagcaagg cagatatcat gagagcttag
     5881 cggcttacag aaaagcaata tcattaagac ctgagctaaa ggaattagac tcgaagaatg
     5941 cccgacttaa aaagatgtat gctgagggga tcctctagag tcgacctgca gctgccgggg
     6001 atcctctaga ggatccgcaa caaggccaac accgctacgg aatgacatat aatctataca
     6061 taaaaatatc atctatgtta ttaattataa tagacatatt aaaaaagttg cgttataaca
     6121 ctttttaagt gatttaattt tgattaaggt tttacatatg agggaagcgg tgatcgccga
     6181 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6241 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6301 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6361 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6421 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6481 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     6541 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     6601 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6661 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6721 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6781 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6841 gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     6901 tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataagtta agcaaccgtt
     6961 ctataatcat aaaaaaagcc tatatagttt gactatatag gcttttttgc tttataatgt
     7021 agttttgaga agcgtcattg cgaggatcct ctagagcaac aaggccaaca ccgctacgga
     7081 atgacatata atctatacat aaaaatatca tctatgttat taattataat agacatatta
     7141 aaaaagttgc gttataacac tttttaagtg atttaatttt gattaaggtt tacatatggt
     7201 atcagaactt atcaaagaga atatgcacat gaaactatac atggaaggta ctgtcaataa
     7261 tcatcatttc aaatgtactt ctgaaggtga gggcaaacct tatgagggta cacaaacaat
     7321 gagaattaag gcagttgaag gcggaccatt gccgtttgct tttgatattc tagctacttc
     7381 gtttatgtat gggagcaaaa cgttcataaa tcatactcaa ggtattccag atttctttaa
     7441 acagtccttt cctgaaggat ttacttggga acgtgtaact acgtatgaag atggtggagt
     7501 attaaccgct acacaagata caagtttaca agacggctgc ttaatctata atgtgaaaat
     7561 acgtggtgta aacttccctt ctaacggacc ggttatgcaa aagaaaaccc tgggatggga
     7621 agcaagtacg gaaactctat atcctgctga tggtggatta gagggtagag cagatatggc
     7681 tttaaagtta gttggtggtg ggcatcttat atgtaatctt aaaacaactt atcgatcaaa
     7741 gaaacccgct aagaatttga aaatgccagg cgtgtattat gtagatagga gattagaaag
     7801 aatcaaagaa gcagacaaag agacatacgt tgaacagcat gaagtagccg ttgcgcgtta
     7861 ttgtgatctt cctagcaaat tagggcatag atgattagtc gacacaatct gccctttcga
     7921 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
     7981 ttacacatgg catggatgag ctctacaaat aaggctaata agcaaccgtt ctataatcat
     8041 aaaaaaagcc tatatagttt gactatatag gcttttttgc tttataatgt agttttgaga
     8101 agcgtcattg cgaggatccc cagcttgcat gcctgcaggc atgcaagctt gagtattcta
     8161 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10321 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10381 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    10441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10561 tatgcggcat cagagcagat tgtactgaga gtgcacctgc ggtgtgaaat accgcacaga
    10621 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    10681 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10741 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10801 gccagtgaat tgtaatacga ctcactata
//
