LOCUS       sequence-162278-        4668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KEAP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_KEAP1_p.D389Y
FEATURES             Location/Qualifiers
     source          1..4668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2068..2167)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2184..2203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2185..2203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2208..2224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2208..2224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2655..3446
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3539..4127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4028..4047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4281..4298
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4457..4484)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4576..4662)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcagcca gatcccaggc ctagcggggc tggggcctgc tgccgattcc
      241 tgcccctgca gtcacagtgc cctgaggggg caggggacgc ggtgatgtac gcctccactg
      301 agtgcaaggc ggaggtgacg ccctcccagc atggcaaccg caccttcagc tacaccctgg
      361 aggatcatac caagcaggcc tttggcatca tgaacgagct gcggctcagc cagcagctgt
      421 gtgacgtcac actgcaggtc aagtaccagg atgcaccggc cgcccagttc atggcccaca
      481 aggtggtgct ggcctcatcc agccctgtct tcaaggccat gttcaccaac gggctgcggg
      541 agcagggcat ggaggtggtg tccattgagg gtatccaccc caaggtcatg gagcgcctca
      601 ttgaattcgc ctacacggcc tccatctcca tgggcgagaa gtgtgtcctc cacgtcatga
      661 acggtgctgt catgtaccag atcgacagcg ttgtccgtgc ctgcagtgac ttcctggtgc
      721 agcagctgga ccccagcaat gccatcggca tcgccaactt cgctgagcag attggctgtg
      781 tggagttgca ccagcgtgcc cgggagtaca tctacatgca ttttggggag gtggccaagc
      841 aagaggagtt cttcaacctg tcccactgcc aactggtgac cctcatcagc cgggacgacc
      901 tgaacgtgcg ctgcgagtcc gaggtcttcc acgcctgcat caactgggtc aagtacgact
      961 gcgaacagcg acggttctac gtccaggcgc tgctgcgggc cgtgcgctgc cactcgttga
     1021 cgccgaactt cctgcagatg cagctgcaga agtgcgagat cctgcagtcc gactcccgct
     1081 gcaaggacta cctggtcaag atcttcgagg agctcaccct gcacaagccc acgcaggtga
     1141 tgccctgccg ggcgcccaag gtgggccgcc tgatctacac cgcgggcggc tacttccgac
     1201 agtcgctcag ctacctggag gcttacaacc ccagtgacgg cacctggctc cggttggcgg
     1261 acctgcaggt gccgcggagc ggcctggccg gctgcgtggt gggcgggctg ttgtacgccg
     1321 tgggcggcag gaacaactcg cccgacggca acacctactc ccgcgccctg gactgttaca
     1381 accccatgac caatcagtgg tcgccctgcg cccccatgag cgtgccccgt aaccgcatcg
     1441 gggtgggggt catcgatggc cacatctatg ccgtcggcgg ctcccacggc tgcatccacc
     1501 acaacagtgt ggagaggtat gagccagagc gggatgagtg gcacttggtg gccccaatgc
     1561 tgacacgaag gatcggggtg ggcgtggctg tcctcaatcg tctcctttat gccgtggggg
     1621 gctttgacgg gacaaaccgc cttaattcag ctgagtgtta ctacccagag aggaacgagt
     1681 ggcgaatgat cacagcaatg aacaccatcc gaagcggggc aggcgtctgc gtcctgcaca
     1741 actgtatcta tgctgctggg ggctatgatg gtcaggacca gctgaacagc gtggagcgct
     1801 acgatgtgga aacagagacg tggactttcg tagcccccat gaagcaccgg cgaagtgccc
     1861 tggggatcac tgtccaccag gggagaatct acgtccttgg aggctatgat ggtcacacgt
     1921 tcctggacag tgtggagtgt tacgacccag atacagacac ctggagcgag gtgacccgaa
     1981 tgacatcggg ccggagtggg gtgggcgtgg ctgtcaccat ggagccctgc cggaagcaga
     2041 ttgaccagca gaactgtacc agttgattgc caactttctt gtacaaagtt ggcattataa
     2101 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2161 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2221 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2281 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2341 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2401 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2461 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2521 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2581 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2641 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2701 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2761 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2821 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2881 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2941 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3001 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3061 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3121 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3181 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3241 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3301 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3361 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3421 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3481 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3661 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4201 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4261 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4321 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4381 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4441 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4501 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4561 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4621 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
