LOCUS       sequence-162282-        5244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK7, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MAPK7_p.T28I
FEATURES             Location/Qualifiers
     source          1..5244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3123..3222)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3239..3258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3240..3258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3263..3279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3263..3279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3710..4501
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4594..5182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5083..5102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccgagc ctctgaagga ggaagacggc gaggacggct ctgcggagcc
      721 ccccgggccc gtgaaggccg aacccgccca catcgctgcc tctgtagcgg ccaagaacct
      781 ggccctgctt aaagcccgct ccttcgatgt gacctttgac gtgggcgacg agtacgagat
      841 catcgagacc ataggcaacg gggcctatgg agtggtgtcc tccgcccgcc gccgcctcac
      901 cggccagcag gtggccatca agaagatccc taatgctttc gatgtggtga ccaatgccaa
      961 gcggaccctc agggagctga agatcctcaa gcactttaaa cacgacaaca tcatcgccat
     1021 caaggacatc ctgaggccca ccgtgcccta tggcgaattc aaatctgtct acgtggtcct
     1081 ggacctgatg gaaagcgacc tgcaccagat catccactcc tcacagcccc tcacactgga
     1141 acacgtgcgc tacttcctgt accaactgct gcggggcctg aagtacatgc actcggctca
     1201 ggtcatccac cgtgacctga agccctccaa cctattggtg aatgagaact gtgagctcaa
     1261 gattggtgac tttggtatgg ctcgtggcct gtgcacctcg cccgctgaac atcagtactt
     1321 catgactgag tatgtggcca cgcgctggta ccgtgcgccc gagctcatgc tctctttgca
     1381 tgagtataca caggctattg acctctggtc tgtgggctgc atctttggtg agatgctggc
     1441 ccggcgccag ctcttcccag gcaaaaacta tgtacaccag ctacagctca tcatgatggt
     1501 gctgggtacc ccatcaccag ccgtgattca ggctgtgggg gctgagaggg tgcgggccta
     1561 tatccagagc ttgccaccac gccagcctgt gccctgggag acagtgtacc caggtgccga
     1621 ccgccaggcc ctatcactgc tgggtcgcat gctgcgtttt gagcccagcg ctcgcatctc
     1681 agcagctgct gcccttcgcc accctttcct ggccaagtac catgatcctg atgatgagcc
     1741 tgactgtgcc ccgccctttg actttgcctt tgaccgcgaa gccctcactc gggagcgcat
     1801 taaggaggcc attgtggctg aaattgagga cttccatgca aggcgtgagg gcatccgcca
     1861 acagatccgc ttccagcctt ctctacagcc tgtggctagt gagcctggct gtccagatgt
     1921 tgaaatgccc agtccctggg ctcccagtgg ggactgtgcc atggagtctc caccaccagc
     1981 cccgccacca tgccccggcc ctgcacctga caccattgat ctgaccctgc agccacctcc
     2041 accagtcagt gagcctgccc caccaaagaa agatggtgcc atctcagaca atactaaggc
     2101 tgcccttaaa gctgccctgc tcaagtcttt gaggagccgg ctcagagatg gccccagcgc
     2161 acccctggag gctcctgagc ctcggaagcc ggtgacagcc caggagcgcc agcgggagcg
     2221 ggaggagaag cggcggaggc ggcaagaacg agccaaggag cgggagaaac ggcggcagga
     2281 gcgggagcga aaggaacggg gggctggggc ctctgggggc ccctccactg accccttggc
     2341 tggactagtg ctcagtgaca atgacagaag cctgttggaa cgctggactc gaatggcccg
     2401 gcccgcagcc ccagccctca cctctgtgcc ggcccctgcc ccagcgccaa cgccaacccc
     2461 aaccccagtc caacctacca gtcctcctcc tggccctgta gcccagccca ctggcccgca
     2521 accacaatct gcgggctcta cctctggccc tgtaccccag cctgcctgcc caccccctgg
     2581 ccctgcaccc caccccactg gccctcctgg gcccatccct gtccccgcgc caccccagat
     2641 tgccacctcc accagcctcc tggctgccca gtcacttgtg ccaccccctg ggctgcctgg
     2701 ctccagcacc ccaggagttt tgccttactt cccacctggc ctgccgcccc cagacgccgg
     2761 gggagcccct cagtcttcca tgtcagagtc acctgatgtc aaccttgtga cccagcagct
     2821 atctaagtca caggtggagg accccctgcc ccctgtgttc tcaggcacac caaagggcag
     2881 tggggctggc tacggtgttg gctttgacct ggaggaattc ttaaaccagt ctttcgacat
     2941 gggcgtggct gatgggccac aggatggcca ggcagattca gcctctctct cagcctccct
     3001 gcttgctgac tggctcgaag gccatggcat gaaccctgcc gatattgagt ccctgcagcg
     3061 tgagatccag atggactccc caatgctgct ggctgacctg cctgacctcc aggaccccta
     3121 gaacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3181 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3241 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3301 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3361 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3421 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3481 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3541 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     3601 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     3661 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     3721 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     3781 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     3841 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     3901 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     3961 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     4021 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4081 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4141 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4201 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4261 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4321 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4381 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4441 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4501 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4561 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4621 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4681 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4741 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4801 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4861 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4921 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4981 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5041 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5101 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5161 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5221 ttgctggcct tttgctcaca tgtt
//
