LOCUS       sequence-162290-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable
            N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cas5c/pHMGWA
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aagcaggctc cgcggccgcc gtactcgagg aaaacctgta ttttcagggc tccttcacca
       61 tgacacatgg ggctgttaag acatacggca tcagactgcg ggtatggggc gactacgcct
      121 gcttcacgcg gccggagatg aaggtggaac gggtgtcata cgatgtcatg cccccttccg
      181 ccgcacgggg catccttgag gccatccact ggaagccggc catcaggtgg attgtggaca
      241 ggattcatgt gctgcggccc atcgtcttcg acaacgtgcg gcgcaacgag gtcagttcga
      301 agatacccaa gcccaatccg gcaacggcca tgcgcgacag gaagccgctc tactttctgg
      361 tcgatgacgg cagcaaccgc cagcaacggg cggcgaccct gctgcgcaac gtcgactatg
      421 tcatcgaagc ccacttcgaa ctgacagaca aggccggagc ggaagacaac gcgggcaagc
      481 atctggacat cttcaggcgt cgtgcgcgcg ccgggcagtc gttccagcag ccatgcctcg
      541 gctgccgcga gtttccggcg tcgttcgaac tgctggaggg ggatgtgccg ctctcgtgct
      601 acgcgggcga gaagcgcgac cttggctaca tgctgctcga catcgacttc gaacgcgaca
      661 tgacgccgct gttcttcaag gccgtcatgg aagacggagt gataacgccc ccgtcgcgca
      721 cgtcaccgga ggtgagggca tgaaagggtg ggc
//
