LOCUS       sequence-162309-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP +52 from TSS; non-template strand; expressed 
            from aTc inducible system in pEZ03 in the NS1 locus with kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas52
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgattacgcc aagcttaatt aagtcaaggg atggagaaag caaaatgaat aaactgttta
       61 cagcagccct tagtggtcta tcatttatcg ttgttcaaca ggcaaaaagt gaacctgtat
      121 ttattgattt tgattaccag tatcggtatg gttatgaata taattacgac tatgatgact
      181 atacctatta ctaccacgcc aatgtctaca cctatagcga tgattcggtt gtattcccag
      241 tcaaagtact cgttaatgct gcatgtcaac aaccacaata ccccattgta ttaggctctg
      301 aagcagatat aagcaacggt acagatgtct tgatcgaaga atctaatgtc attattgata
      361 atgatttcag tacaaattgc tctgctttta cagaaaaaaa tgtcctgcta ctctgggaag
      421 aaaacgagat aaaataggcg tcccttctgg tttttctttc tgtaaagctg tactgtttaa
      481 aaatagatct taaattatag aagcactttg actttatttt cggatccacc ttttcgggga
      541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      601 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
      661 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
      721 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
      781 atcaatacaa cctattaatt tcccctcgtc aaa
//
