LOCUS       sequence-162324-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Palm4-12GS-I3-01-myc-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-10
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             808..837
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cgggccctct agaattatcg atccggaggt
      121 accatggatt gtttgtgtat cgttaccacc aagaagtacc gtggttctaa atctggctcc
      181 ggctctgact ctggtagcaa gatcgaagag ctgttcaaga aacacaagat cgttgccgtg
      241 ctgcgtgcca atagtgtgga agaagcgaaa aagaaagcgc tggcggtttt cctgggcggc
      301 gttcatctga ttgaaattac ctttaccgtg ccggatgcgg ataccgtgat taaggaactg
      361 agctttctga aggaaatggg cgcgattatt ggtgcgggca ccgtgaccag cgtggagcag
      421 tgccgtaaag cggtggaaag tggcgccgaa ttcattgtga gtccgcacct ggacgaggaa
      481 attagccaat tttgcaagga gaagggtgtg ttctatatgc caggcgttat gaccccgacc
      541 gaactggtga aagccatgaa actgggccat accatcttaa aactgtttcc gggtgaggtg
      601 gtgggtccgc agtttgttaa agcgatgaaa ggtccgtttc cgaatgtgaa atttgtgcca
      661 accggcggtg ttaatctgga caatgtgtgc gaatggttca aagcgggcgt gctggccgtg
      721 ggcgtgggca gcgcgttagt gaaaggcacc ccggtggaag tggcggaaaa ggccaaggcg
      781 ttcgttgaga agattcgtgg ctgcaccgaa cagaaactga ttagcgaaga agatctgcag
      841 tctcgtccgg aaccgaccgc accgccggaa gaatctttcc gttctggtgt tgaaaccacc
      901 acgccaccgc aaaaacagga gccgatcgac aaagaactgt acccgctgac ctctctgcgt
      961 tctctgttcg gtaacgaccc gtcttctcag tg
//
