LOCUS       sequence-162325-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Palm4-12GS-I3-01-myc-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-10
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..176)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(919..938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(920..938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacgggtcgt taccgaacag agaacgcaga gaggtcagcg ggtacagttc tttgtcgatc
       61 ggctcctgtt tttgcggtgg cgtggtggtt tcaacaccag aacggaaaga ttcttccggc
      121 ggtgcggtcg gttccggacg agactgcaga tcttcttcgc taatcagttt ctgttcggtg
      181 cagccacgaa tcttctcaac gaacgccttg gccttttccg ccacttccac cggggtgcct
      241 ttcactaacg cgctgcccac gcccacggcc agcacgcccg ctttgaacca ttcgcacaca
      301 ttgtccagat taacaccgcc ggttggcaca aatttcacat tcggaaacgg acctttcatc
      361 gctttaacaa actgcggacc caccacctca cccggaaaca gttttaagat ggtatggccc
      421 agtttcatgg ctttcaccag ttcggtcggg gtcataacgc ctggcatata gaacacaccc
      481 ttctccttgc aaaattggct aatttcctcg tccaggtgcg gactcacaat gaattcggcg
      541 ccactttcca ccgctttacg gcactgctcc acgctggtca cggtgcccgc accaataatc
      601 gcgcccattt ccttcagaaa gctcagttcc ttaatcacgg tatccgcatc cggcacggta
      661 aaggtaattt caatcagatg aacgccgccc aggaaaaccg ccagcgcttt ctttttcgct
      721 tcttccacac tattggcacg cagcacggca acgatcttgt gtttcttgaa cagctcttcg
      781 atcttgctac cagagtcaga gccggagcca gatttagaac cacggtactt cttggtggta
      841 acgatacaca aacaatccat ggtacctccg gatcgataat tctagagggc ccgtttaaac
      901 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      961 tgg
//
