LOCUS       sequence-162330-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I3-01p-myc-Late1-CaaX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-12
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(192..221)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(892..911)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(893..911)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataattacac actttgtctt tgacttcttt ttcttcttct gagaagacgg gtcgttaccg
       61 aacagagaac gcagagaggt cagcgggtac agttctttgt cgatcggctc ctgtttttgc
      121 ggtggcgtgg tggtttcaac accagaacgg aaagattctt ccggcggtgc ggtcggttcc
      181 ggacgagact gcagatcttc ttcgctaatc agtttctgtt cggtgcagcc acgaatcttc
      241 tcaacgaacg ccttggcctt ttccgccact tccaccgggg tgcctttcac taacgcgctg
      301 cccacgccca cggccagcac gcccgctttg aaccattcgc acacattgtc cagattaaca
      361 ccgccggttg gcacaaattt cacattcgga aacggacctt tcatcgcttt aacaaactgc
      421 ggacccacca cctcacccgg aaacagtttt aagatggtat ggcccagttt catggctttc
      481 accagttcgg tcggggtcat aacgcctggc atatagaaca cacccttctc cttgcaaaat
      541 tggctaattt cctcgtccag gtgcggactc acaatgaatt cggcgccact ttccaccgct
      601 ttacggcact gctccacgct ggtcacggtg cccgcaccaa taatcgcgcc catttccttc
      661 agaaagctca gttccttaat cacggtatcc gcatccggca cggtaaaggt aatttcaatc
      721 agatgaacgc cgcccaggaa aaccgccagc gctttctttt tcgcttcttc cacactattg
      781 gcacgcagca cggcaacgat cttgtgtttc ttgaacagct cttcgatctt catggtacct
      841 ccggatcgat aattctagag ggcccgttta aacgctagcc agcttgggtc tccctatagt
      901 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
      961 tgcttatata ga
//
