LOCUS       sequence-162333-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Late2-4GS-I3-01-myc-PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-20
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             807..836
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgtttaaac gggccctcta gaattatcga tccggaggta
      121 ccatgcgccg cgtgatcctg ccgaccgcgc cgccggaata tatggaagcg atttacccag
      181 ttcgcggtag cggtagcaag atcgaagagc tgttcaagaa acacaagatc gttgccgtgc
      241 tgcgtgccaa tagtgtggaa gaagcgaaaa agaaagcgct ggcggttttc ctgggcggcg
      301 ttcatctgat tgaaattacc tttaccgtgc cggatgcgga taccgtgatt aaggaactga
      361 gctttctgaa ggaaatgggc gcgattattg gtgcgggcac cgtgaccagc gtggagcagt
      421 gccgtaaagc ggtggaaagt ggcgccgaat tcattgtgag tccgcacctg gacgaggaaa
      481 ttagccaatt ttgcaaggag aagggtgtgt tctatatgcc aggcgttatg accccgaccg
      541 aactggtgaa agccatgaaa ctgggccata ccatcttaaa actgtttccg ggtgaggtgg
      601 tgggtccgca gtttgttaaa gcgatgaaag gtccgtttcc gaatgtgaaa tttgtgccaa
      661 ccggcggtgt taatctggac aatgtgtgcg aatggttcaa agcgggcgtg ctggccgtgg
      721 gcgtgggcag cgcgttagtg aaaggcaccc cggtggaagt ggcggaaaag gccaaggcgt
      781 tcgttgagaa gattcgtggc tgcaccgaac agaagctgat ctccgaagaa gacctgcacg
      841 gtctgcagga cgacccggac ctgcaagcac tgctcaaggg cagccagctg ctgaaagtta
      901 aatcttcttc ttggcgtc
//
