LOCUS       sequence-162341-        4863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pACSA_Pcpt_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ01
FEATURES             Location/Qualifiers
     source          1..4863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      154..742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     643..662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     896..913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1030..1051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1066..1071,1072..1089,1090..1096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1104..1120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1109..1131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1128..1144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1128..1144
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1162..1180
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1162..1179
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2600..2618)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2614..2661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3165..3184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3166..3184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3191..3208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3191..3207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3200..3222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3348..3803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3435..3454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3645..3666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3881..3985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3986..4054,4055..4846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPEMNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4204..4223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
       61 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
      121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      301 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      541 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      901 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      961 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     1021 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     1081 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     1141 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgaggtga
     1201 ctgccgcact caatgttcaa ccagatctag tgattatttc ggggttagca gccgatgcgg
     1261 gcaacctggt gaagcaactg cgagaattag gttacaacgg cattattgtt ggggggaacg
     1321 gcttaaatac ttctaatatt ttccccgtct gccaagcaaa atgtgatggg gtgttggtgg
     1381 cccaagccta cagtgccgag ttagataatg agattaaccg cgcgtttcgg gacgcctatt
     1441 ttcaacaaaa ccaaaaagag ccgccccaat ttagtgccca ggcttttacg gcgatccaag
     1501 tttttgttga agccctcagc agcctcgatg aaaaaacgcc cttagaaact cttgctctac
     1561 cggacttgcg acgacaactg cgggacgaaa tttttgcagg tacctacgtc acgcctttgg
     1621 gtgaaatttc cttcacagag gaaggggaaa ttgtccagaa ggaatttttt gtggcccaaa
     1681 ttgaaatgga tgaatcgggt caacaggggc gtttcgcctt cattgaaacg aacaaaggaa
     1741 ttcttaacaa aaaagcagga ataaaattaa caagatgtaa cagacataag tcccatcacc
     1801 gttgtataaa gttaactgtg ggattgcaaa aggagctccc aactcataaa gtcaagtagg
     1861 agattaatca tatgtcggtc atcaagcccg aaatgaaaat taaactgcgc atggagggcg
     1921 ctgtgaacgg gcacaagttc gtgatcgaag gtgaaggcat tggtaagccg tatgaaggta
     1981 ctcaaaccct ggacttaacg gttaaagaag gtgccccgtt gccatttagc tacgacatcc
     2041 tgacacccgc ttttcagtat ggcaatcgcg cgttcaccaa gtatccgaaa gatattccgg
     2101 attatttcaa gcaggcattc ccggaaggat actcatggga acgctcgatg acctacgaag
     2161 accagggcat ttgcattgcc acaagcgaca tcacaatgga gggtgattgc tttttttaca
     2221 aaattcgctt cgacgggaca aacttcccgc cgaatggccc agtgatgcag aaaaagacgc
     2281 ttaaatggga accgtcgaca gagaaaatgt acgttcgcga tggcgttctg aaaggcgatg
     2341 ttaatatggc gctgcttctc gaagggggag gccattaccg ttgtgatttc aaaaccacat
     2401 acaaagcaaa aaaagatgtt cgtttgccgg acgcgcatga agtcgatcac cgcattgaga
     2461 tcctgtcaca tgataaagat tacaataagg tccgccttta tgagcacgct gaggcccgct
     2521 attctatgct gcccagccaa gctaaataag gatccggctg ctaacaaagc ccgaaaggaa
     2581 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     2641 cgggtcttga cgggtttttt gtctagagcg ctcaagttga tcgaatgtat gccccggata
     2701 tgacctaggg agattcttct aaagcgagca gggtttgcca agtggcgatc gcctcaggga
     2761 accataacca actttgttcg aggcggcggg ggttgagctg ggaacgatcg agatctaggg
     2821 cgatcgcccg gagtttttcg gctttcccta caagaatttc ctggtcaacg tcggtgggtt
     2881 gcctttgggc caaatcgtac atactaatcg ccaggcccgc ataaatctcc gcctggaggg
     2941 ctggggggaa atcctgggtc gttgccgccg ctaaggcttc ataccaacga tcattggcgg
     3001 cttcgtaatc tgcattgagg taatggacaa aacccagggc catcaaaatt tcggggtcgt
     3061 tgggctgttg ggctagggcc atgtcccaat tgtcttgcac cgtggcgatc gccgttgctt
     3121 gggttggcga tggtgggacg gcagccaaag ttgttcagca tgcgccctat agtgagtcgt
     3181 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     3361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3781 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     3841 atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3901 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3961 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4021 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4081 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4141 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4201 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4261 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4321 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4381 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4441 catgggggat catgtaactc gccttgatcg ttgggaaccg gagatgaatg aagccatacc
     4501 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4561 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4621 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4681 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4741 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4801 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4861 tta
//
