LOCUS       sequence-162345-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Late12-I3-01-myc-PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-22
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             893..922
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta actagagaac ccactgcctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acgggccctc tagaattatc gatccggagg
      121 taccatgctg cagtctcgtc cggaaccgac cgcaccgccg gaagaatctt tccgttctgg
      181 tgttgaaacc accacgccac cgcaaaaaca ggagccgatc gacaaagaac tggcggcgct
      241 gacctctctg cgttctctgt tcggtaacga cccgtcttct cagaagatcg aagagctgtt
      301 caagaaacac aagatcgttg ccgtgctgcg tgccaatagt gtggaagaag cgaaaaagaa
      361 agcgctggcg gttttcctgg gcggcgttca tctgattgaa attaccttta ccgtgccgga
      421 tgcggatacc gtgattaagg aactgagctt tctgaaggaa atgggcgcga ttattggtgc
      481 gggcaccgtg accagcgtgg agcagtgccg taaagcggtg gaaagtggcg ccgaattcat
      541 tgtgagtccg cacctggacg aggaaattag ccaattttgc aaggagaagg gtgtgttcta
      601 tatgccaggc gttatgaccc cgaccgaact ggtgaaagcc atgaaactgg gccataccat
      661 cttaaaactg tttccgggtg aggtggtggg tccgcagttt gttaaagcga tgaaaggtcc
      721 gtttccgaat gtgaaatttg tgccaaccgg cggtgttaat ctggacaatg tgtgcgaatg
      781 gttcaaagcg ggcgtgctgg ccgtgggcgt gggcagcgcg ttagtgaaag gcaccccggt
      841 ggaagtggcg gaaaaggcca aggcgttcgt tgagaagatt cgtggctgca ccgaacagaa
      901 gctgatctcc gaagaagacc tgcacggtct gcaggacgac ccggacctgc aagcactgct
      961 ca
//
