LOCUS       sequence-162361-        9151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2A
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(11..45)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     CDS             join(175..201,202..204,205..2691,2692..2694,2695..4281)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(4317..4335)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4331..4378
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4882..4901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4883..4901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4908..4925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4908..4924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4917..4939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5065..5520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5152..5171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5362..5383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5598..5702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5703..5771,5772..6563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPEMNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5921..5940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6734..7322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7223..7242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7476..7493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7610..7631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7646..7651,7652..7669,7670..7676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7684..7700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7689..7711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7708..7724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7708..7724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7742..7760
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7742..7759
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(8320..8347)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(8363..8434)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8482..8514)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     CDS             complement(8515..9135)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     9043..9062
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             join(9142..9151,1..2)
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttctctagta gctagcataa tccctaggac tgagctagcc atcaggttaa caaaaaagca
       61 ggaatatccc tatcagtgat agacagacat ccctatcagt gatagagtat aaagttaact
      121 gtgggattgc aaaaggagct cccaactcat aaagtcaagt aggagattaa ttccatggat
      181 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact
      241 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
      301 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
      361 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
      421 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
      481 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
      541 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
      601 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
      661 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
      721 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
      781 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
      841 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gattatttgg gaatctcatt
      901 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
      961 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
     1021 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
     1081 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt
     1141 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
     1201 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
     1261 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
     1321 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa
     1381 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
     1441 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
     1501 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
     1561 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
     1621 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
     1681 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
     1741 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt
     1801 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
     1861 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
     1921 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa
     1981 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca
     2041 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc
     2101 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg
     2161 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt
     2221 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg
     2281 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa
     2341 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa
     2401 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa
     2461 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa
     2521 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa
     2581 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg
     2641 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt
     2701 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa
     2761 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
     2821 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa
     2881 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
     2941 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
     3001 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
     3061 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
     3121 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca
     3181 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
     3241 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc
     3301 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
     3361 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca
     3421 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca
     3481 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt
     3541 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
     3601 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
     3661 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
     3721 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt
     3781 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa
     3841 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
     3901 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
     3961 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
     4021 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
     4081 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct
     4141 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt
     4201 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg
     4261 agtcagctag gaggtgacta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     4321 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgacgg gttttttgtc
     4381 tagagcgctc aagttgatcg aatgtatgcc ccggatatga cctagggaga ttcttctaaa
     4441 gcgagcaggg tttgccaagt ggcgatcgcc tcagggaacc ataaccaact ttgttcgagg
     4501 cggcgggggt tgagctggga acgatcgaga tctagggcga tcgcccggag tttttcggct
     4561 ttccctacaa gaatttcctg gtcaacgtcg gtgggttgcc tttgggccaa atcgtacata
     4621 ctaatcgcca ggcccgcata aatctccgcc tggagggctg gggggaaatc ctgggtcgtt
     4681 gccgccgcta aggcttcata ccaacgatca ttggcggctt cgtaatctgc attgaggtaa
     4741 tggacaaaac ccagggccat caaaatttcg gggtcgttgg gctgttgggc tagggccatg
     4801 tcccaattgt cttgcaccgt ggcgatcgcc gttgcttggg ttggcgatgg tgggacggca
     4861 gccaaagttg ttcagcatgc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     4921 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4981 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5041 ttgtgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5101 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5161 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5221 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5281 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5341 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5401 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5461 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5521 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
     5581 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5701 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5761 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5821 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5881 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5941 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6001 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6061 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6121 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6181 ttgatcgttg ggaaccggag atgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6241 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6301 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6361 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6421 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6481 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6601 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7441 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7501 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7561 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7621 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7681 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7741 tatttaggtg acactataga atactcaagc tatgaggtga ctgccgcact caatgttcaa
     7801 ccagatctag tgattatttc ggggttagca gccgatgcgg gcaacctggt gaagcaactg
     7861 cgagaattag gttacaacgg cattattgtt ggggggaacg gcttaaatac ttctaatatt
     7921 ttccccgtct gccaagcaaa atgtgatggg gtgttggtgg cccaagccta cagtgccgag
     7981 ttagataatg agattaaccg cgcgtttcgg gacgcctatt ttcaacaaaa ccaaaaagag
     8041 ccgccccaat ttagtgccca ggcttttacg gcgatccaag tttttgttga agccctcagc
     8101 agcctcgatg aaaaaacgcc cttagaaact cttgctctac cggacttgcg acgacaactg
     8161 cgggacgaaa tttttgcagg tacctacgtc acgcctttgg gtgaaatttc cttcacagag
     8221 gaaggggaaa ttgtccagaa ggaatttttt gtggcccaaa ttgaaatgga tgaatcgggt
     8281 caacaggggc gtttcgcctt cattgaaacg aactataaac gcagaaaggc ccacccgaag
     8341 gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata aaacgaaagg
     8401 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggctctagta gtgatctaca
     8461 ctagcactat cagtgttatt aagctactaa agcgtagttt tcgtcgtttg cagcggaccc
     8521 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
     8581 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
     8641 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
     8701 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
     8761 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
     8821 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
     8881 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
     8941 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
     9001 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
     9061 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
     9121 atctaatctg gacatctagt atttctcctc t
//
