LOCUS       sequence-162362-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C1-10GS-I3-01-myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-27
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..949
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag cgtttaaacg ggccctctag aattatcgat ccggaggtac
      121 catgccgcat cgttttaaag tgcataatta catgagcccg accttttgcg atcattgcgg
      181 cagcctgctg tggggcttag tgaaacaggg cctgaaatgc gaagattgcg gtatgaatgt
      241 gcatcataaa tgccgtgaaa aagtggcgaa tctgtgcggc ggcagcgacg gctccggccg
      301 ttccggttcc aagatcgaag agctgttcaa gaaacacaag atcgttgccg tgctgcgtgc
      361 caatagtgtg gaagaagcga aaaagaaagc gctggcggtt ttcctgggcg gcgttcatct
      421 gattgaaatt acctttaccg tgccggatgc ggataccgtg attaaggaac tgagctttct
      481 gaaggaaatg ggcgcgatta ttggtgcggg caccgtgacc agcgtggagc agtgccgtaa
      541 agcggtggaa agtggcgccg aattcattgt gagtccgcac ctggacgagg aaattagcca
      601 attttgcaag gagaagggtg tgttctatat gccaggcgtt atgaccccga ccgaactggt
      661 gaaagccatg aaactgggcc ataccatctt aaaactgttt ccgggtgagg tggtgggtcc
      721 gcagtttgtt aaagcgatga aaggtccgtt tccgaatgtg aaatttgtgc caaccggcgg
      781 tgttaatctg gacaatgtgt gcgaatggtt caaagcgggc gtgctggccg tgggcgtggg
      841 cagcgcgtta gtgaaaggca ccccggtgga agtggcggaa aaggccaagg cgttcgttga
      901 gaagattcgt ggctgcaccg aacagaagct gatctccgaa gaagacctgt gactcgagaa
      961 gcttgctagc agatctt
//
