LOCUS       sequence-162364-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23113_tetR PEZ4_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ12
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..74
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(83..117)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct ggacatctag
       61 tatttctcct ctttctctag tagctagcat aatccctagg actgagctag ccatcaggtt
      121 aacaaaaaag caggaatatc cctatcagtg atagacagac atccctatca gtgatagata
      181 taatgttaac tgtgggattg caaaaggagc tcccaactca taaagtcaag taggagatta
      241 atcatatgtc ggtcatcaag cccgaaatga aaattaaact gcgcatggag ggcgctgtga
      301 acgggcacaa gttcgtgatc gaaggtgaag gcattggtaa gccgtatgaa ggtactcaaa
      361 ccctggactt aacggttaaa gaaggtgccc cgttgccatt tagctacgac atcctgacac
      421 ccgcttttca gtatggcaat cgcgcgttca ccaagtatcc gaaagatatt ccggattatt
      481 tcaagcaggc attcccggaa ggatactcat gggaacgctc gatgacctac gaagaccagg
      541 gcatttgcat tgccacaagc gacatcacaa tggagggtga ttgctttttt tacaaaattc
      601 gcttcgacgg gacaaacttc ccgccgaatg gcccagtgat gcagaaaaag acgcttaaat
      661 gggaaccgtc gacagagaaa atgtacgttc gcgatggcgt tctgaaaggc gatgttaata
      721 tggcgctgct tctcgaaggg ggaggccatt accgttgtga tttcaaaacc acatacaaag
      781 caaaa
//
