LOCUS       sequence-162374-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I3-01p-myc-Late1-PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-25
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             719..748
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
       61 gctagcgttt aaacgggccc tctagaatta tcgatccgga ggtaccatga agatcgaaga
      121 gctgttcaag aaacacaaga tcgttgccgt gctgcgtgcc aatagtgtgg aagaagcgaa
      181 aaagaaagcg ctggcggttt tcctgggcgg cgttcatctg attgaaatta cctttaccgt
      241 gccggatgcg gataccgtga ttaaggaact gagctttctg aaggaaatgg gcgcgattat
      301 tggtgcgggc accgtgacca gcgtggagca gtgccgtaaa gcggtggaaa gtggcgccga
      361 attcattgtg agtccgcacc tggacgagga aattagccaa ttttgcaagg agaagggtgt
      421 gttctatatg ccaggcgtta tgaccccgac cgaactggtg aaagccatga aactgggcca
      481 taccatctta aaactgtttc cgggtgaggt ggtgggtccg cagtttgtta aagcgatgaa
      541 aggtccgttt ccgaatgtga aatttgtgcc aaccggcggt gttaatctgg acaatgtgtg
      601 cgaatggttc aaagcgggcg tgctggccgt gggcgtgggc agcgcgttag tgaaaggcac
      661 cccggtggaa gtggcggaaa aggccaaggc gttcgttgag aagattcgtg gctgcaccga
      721 acagaaactg attagcgaag aagatctgca gtctcgtccg gaaccgaccg caccgccgga
      781 agaatctttc cgttctggtg ttgaaaccac cacgccaccg caaaaacagg agccgatcga
      841 caaagaactg tacccgctga cctctctgcg ttctctgttc ggtaacgacc cgtcttctca
      901 gcacggtctg caggacgacc cggacctgca agcactgctc aagggcagcc agctgctgaa
      961 agttaaatct tc
//
