LOCUS       sequence-162377-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I3-01p-myc-10GS-PH-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-26
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             727..756
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagctggc tagcgtttaa acgggccctc tagaattatc gatccggagg taccatgaag
      121 atcgaagagc tgttcaagaa acacaagatc gttgccgtgc tgcgtgccaa tagtgtggaa
      181 gaagcgaaaa agaaagcgct ggcggttttc ctgggcggcg ttcatctgat tgaaattacc
      241 tttaccgtgc cggatgcgga taccgtgatt aaggaactga gctttctgaa ggaaatgggc
      301 gcgattattg gtgcgggcac cgtgaccagc gtggagcagt gccgtaaagc ggtggaaagt
      361 ggcgccgaat tcattgtgag tccgcacctg gacgaggaaa ttagccaatt ttgcaaggag
      421 aagggtgtgt tctatatgcc aggcgttatg accccgaccg aactggtgaa agccatgaaa
      481 ctgggccata ccatcttaaa actgtttccg ggtgaggtgg tgggtccgca gtttgttaaa
      541 gcgatgaaag gtccgtttcc gaatgtgaaa tttgtgccaa ccggcggtgt taatctggac
      601 aatgtgtgcg aatggttcaa agcgggcgtg ctggccgtgg gcgtgggcag cgcgttagtg
      661 aaaggcaccc cggtggaagt ggcggaaaag gccaaggcgt tcgttgagaa gattcgtggc
      721 tgcaccgaac agaagctgat ctccgaagaa gacctgggca gcgacggctc cggccgttcc
      781 ggttcccacg gtctgcagga cgacccggac ctgcaagcac tgctcaaggg cagccagctg
      841 ctgaaagtta aatcttcttc ttggcgtcgt gaacgtttct acaaactcca ggaggactgc
      901 aaaaccatct ggcaggaatc ccgtaaagtt atgcgttctc cggaatctca gctgttctct
      961 atcgaa
//
