LOCUS       sequence-162389-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NUP88, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_NUP88_WT_V5
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2895..2994)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3011..3030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3012..3030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3035..3051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3482..4273
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4366..4954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4855..4874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcggccg ccgagggacc ggtgggcgac ggcgagctgt ggcagacctg
      721 gcttcctaac cacgtcgtgt tcttgcggct ccgggaggga ctgaaaaacc agagtccaac
      781 cgaagctgag aaaccagctt cttcgtcgtt gccttcgtcg ccgccgccgc agttgctgac
      841 gagaaacgtg gtctttggcc tcggcggaga gcttttcctg tgggacggag aagacagctc
      901 cttcttagtc gttcgccttc ggggccccag cggcggcggc gaagagcccg ccctgtccca
      961 gtaccagaga ttgctttgca taaatccacc cctgtttgaa atctatcaag tcttgttaag
     1021 cccaacacaa catcatgtag cacttatagg aataaaagga cttatggtat tagaattacc
     1081 taaaagatgg gggaagaatt ctgaatttga aggtggaaaa tcaacagtga attgtagtac
     1141 cactccagtt gcggagagat ttttcaccag ttccacctct ctgactctaa agcatgctgc
     1201 atggtatcca agtgaaatcc tggatcccca cgtagtgctg ttaacatcag acaacgtaat
     1261 cagaatttac tcactacgtg agccgcagac acccactaac gtgataatac tttcagaagc
     1321 cgaagaggaa agtctagtac tcaataaagg aagggcgtat accgcatctc taggagagac
     1381 agcagttgca tttgactttg ggccattggc agcagtccca aagactctat ttggacaaaa
     1441 cggcaaagat gaagtagtgg catacccact gtacatctta tatgaaaatg gagagacttt
     1501 cctgacatac atcagtctgt tacacagccc tggaaatatt ggaaagctgt tgggtccatt
     1561 gcccatgcat cctgcggctg aagataacta tggttatgat gcgtgtgctg tactctgctt
     1621 accctgtgtc cccaatatct tagtgatcgc tactgaatca ggaatgctgt atcactgtgt
     1681 cgtgctagaa ggggaagaag aagatgacca cacgtcagaa aagtcctggg attccaggat
     1741 tgacctcatt ccttctctgt atgtgtttga atgtgttgag ttggagcttg ctttgaaact
     1801 ggcatctgga gaggatgacc cttttgattc tgacttttct tgtccagtca aacttcatag
     1861 agatcccaag tgtccttcaa gatatcactg tactcatgaa gctggtgtac atagtgttgg
     1921 gctaacttgg attcataaac ttcacaaatt tcttggatca gatgaagaag ataaggatag
     1981 tttacaggaa ctctctacag aacagaaatg ctttgttgaa cacatccttt gtacgaagcc
     2041 attgccctgc aggcagccag ctccaattcg aggattttgg attgtacctg acattctggg
     2101 acccacgatg atctgcatca ccagtaccta tgaatgcctc atatggccgt tattaagtac
     2161 agtccatcca gcgtctcctc ccctgctttg tactcgagaa gatgttgaag tggcagagtc
     2221 tcccctccgt gttctggctg aaaccccaga ttcctttgaa aagcatatta gaagcatttt
     2281 gcaacgtagt gttgccaatc cagcattttt gaaagcttct gaaaaggaca tagcccctcc
     2341 tcctgaagaa tgccttcagc tcctcagcag agccacccag gtgttcagag agcagtacat
     2401 tctcaaacag gacttggcaa aggaggagat tcagcggagg gtcaaattat tatgtgacca
     2461 aaaaaagaaa caactagaag atctcagtta ttgtcgagaa gagaggaaaa gtctgcggga
     2521 aatggctgag cgtttagctg acaaatatga ggaagctaaa gaaaaacaag aggatatcat
     2581 gaacaggatg aaaaaactac ttcacagttt tcactctgag ctcccagttc tctctgatag
     2641 tgagcgagac atgaagaaag aattacagct gatacctgat caacttcgac atttgggcaa
     2701 tgccatcaaa caggttacta tgaaaaagga ttatcaacag caaaagatgg agaaggtgtt
     2761 gagtcttcca aaacccacca ttattctcag tgcctaccag cgaaagtgca ttcagtccat
     2821 cctgaaagag gagggtgaac atataaggga aatggtgaag caaatcaatg atatccgcaa
     2881 tcatgtaaac ttcttgccaa ctttcttgta caaagttggc attataagaa agcattgctt
     2941 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     3001 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3061 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     3121 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     3181 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     3241 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     3301 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     3361 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     3421 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     3481 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     3541 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     3601 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     3661 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     3721 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     3781 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3841 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3901 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3961 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     4021 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     4081 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     4141 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     4201 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     4261 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     4321 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4381 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4441 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4501 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4561 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4621 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4681 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4741 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4801 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4861 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4921 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4981 gttcctggcc ttttgctggc cttttgctca catgtt
//
