LOCUS       sequence-162393-        5013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DAXX, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DAXX_WT_V5
FEATURES             Location/Qualifiers
     source          1..5013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1302..1401)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1417..1434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1417..1433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1426..1448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1497..1583
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1675..1702
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1861..1878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2032..2620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2112..2131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2713..3504)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3935..3951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3935..3951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3956..3975
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3956..3974
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3992..4091
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agctctggag gcagaagggc acccctggga tgccattcca ctagggccct caccagaatc
       61 caaggaggct tcgggggtgt ctgcagagtg ggaagaggtg ccttggagcc gagctcttct
      121 cttttttctc tcgccctcct cacttttgtc ttgcaacatt gcatatttgg agatgacctc
      181 atccagccga ctcatggcca aactccggtt ttcccgaagg cgccgggcca acacaggatc
      241 tgatagtgca gggtcaacgc ctggcctata gtcatctgtg aggtggcagc caaagttgta
      301 gatgagatcg aggtgacgtc gctcctgtaa cctgatgccc acatctcgga aggcatcctg
      361 agccatgagc tggagctgct gtcgggggag gccaaggctg tgtcgggcag ctgccttctc
      421 tacagcccga agcacatccc catagtcagg gaaggtatca ggccctggct tgttgatgag
      481 ccgctcaatg cgcctgttaa cctctgggta gcgggtgcca cggtagggga tgcgctgctc
      541 tatgacacgg ccggtcagtg aagagcagtc tttcagctca catagtcgcc caaagaggcg
      601 gatcagctta cgcttcaacc gtgcctcctg caggtatgcg gagtctgggt catccaattc
      661 tgagagatcc aactcctttt cctgcagccg ccggatctct gccacataga gcgccagcag
      721 ctgctccaaa cgctggatct gccgccggga accacgggtc cttggagact gagaggcagt
      781 gttttcagca tttgtggggt ccaaggagag gtgtgtggga gggttattcc cagagggctc
      841 attggaggtg gtggcggcag gggccaagtt cagcttcttt ttggctgagt gggccttgag
      901 aacagtgcag agctcattga tgtagacata gagcttggct ggccggctcc gggcccgaga
      961 caggacccta gagaggatgt tgcagaactc cgccgaggcc aaaaacagag agtgggcacg
     1021 ttgctgccgg ttatagagga atgggaccac ctcagggtgg tctgctgtct gcatcttaca
     1081 aagttcaagg aactcttcga acagcttctc attctccagc ttgtagcatt tcttgccgcc
     1141 cgaactactg cttcctctgg ccccatgagg ctcagaggag ctaggggctt ctgccccagg
     1201 tgaggccgca ttggggagtg ggtgggaggg ccctggctga gcagctgctt catcttcgtc
     1261 atcatcatcc agcacgatga tgctgttagc ggtggccatg gtgccaactt ttttgtacaa
     1321 agttggcatt ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag
     1381 tcaaaataaa atcattattt ggggcccgag cttaagactg gccgtcgttt tacaacgtcg
     1441 tgactgggaa aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa
     1501 taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1561 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc
     1621 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg
     1681 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctggctag cggtacgcgt
     1741 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1801 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2461 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2641 tacggggtct gacgctcagt ggaacgacgc gtaactcacg ttaagggatt ttggtcatgg
     2701 gtggctcgag ggttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     2761 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     2821 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     2881 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     2941 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     3001 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     3061 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     3121 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     3181 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     3241 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     3301 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     3361 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     3421 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     3481 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
     3541 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     3601 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
     3661 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     3721 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
     3781 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
     3841 atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt gtgcaatgta
     3901 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
     3961 cgactcacta taggggatat cagctggatg gcaaataatg attttatttt gactgatagt
     4021 gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa
     4081 gaaagttggc aaatcagagt ctgagagcac gatgatctct tctggatcgc attgtgtggc
     4141 cacacttgtc ttgcaagtac caggccgagg aggctgtgaa tggggggttt gggacagccg
     4201 ggctggagaa gggatgcaga gggagctggt caccaggcca tggctgggag agtccaccct
     4261 cgtggaggaa tcagcaactg gggccaagga agccaagggg gaaggtgggc tgggcagggt
     4321 acatatcttt ttcccattct tctcatgcac tgacctttgc ctttccacat agctgtttcc
     4381 taatggccct gatcctgttt gcttcttctc cttccgagat tttttgcagg ggggaccaga
     4441 atctccccag ttgtgaggag agacgcctcc attgaaggaa gtagaagaga ccatgcctgc
     4501 tccattctct aagacagtgg tgaagggctc ctctgattgc ttcctggaag aggaaatgtc
     4561 cgtctccaca gaggaagggg tatccagggg caaagcttca atctctagct caaagagctg
     4621 agacacaggg ctttcttcct ccagggtcag ctcctcaggc tgttctccat tgctttcagc
     4681 atctatgctg gagggggcca ggggttcttc tgacagtaac gatggtgaca ctatgcgtcc
     4741 tttgttttgc tgctcccctg aagatctgct gatctgtttg ccaggttcca ggttcttttc
     4801 attggagatc tgtagtgagg acatggggct cttgtctcca tctttacctg ctgctgcttc
     4861 ttcctcttcg tcctcctctt catcatcctc ctgaccctcc tgcatctgtt ccagatcctc
     4921 ctcctcttca gaatctgtgg cctcctcctc ttcttcttcc tcctcctcct cctcttcctc
     4981 atcactctcc tcatcgtctt cgtcatctgt ctc
//
