LOCUS       sequence-162396-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-cul4
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1433..1450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1434..1450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1458..1478
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1726..1750
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2211..2231)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2979..3007
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(3169..3193)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3683..3703)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5769..5780
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(7416..8004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7496..7515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8175..8966,8967..9035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8798..8817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9036..9140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9208..9226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9264..9286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 atctaaatac tgaacttttt gtgtaatctt tagtgcagaa aaatgaccaa aatcagcgat
       61 tttccgatta tacccaaaat atctcagctc aatgagcacc tataaaaatg taaaaataaa
      121 gaaagtagtc gaaccatatt ttgtaaaggc taaaaaactg aataaatttt tatccatcca
      181 gcgcggcgtt acaatccgtg gcccatgtca cgcacggctt tcaaagaaaa aaaaacagtc
      241 tgtttgcgcg acatcgatct ggcaaaaatc ctaaaaacga ctatgcgtgg ctcactttat
      301 attaatatga aaaatacaaa cttggtggtg caggtgctgc agaggccatc gatgaagaag
      361 tggaggcctt gttgtcgatc tacaaaattg tatggttgtt tgagattttc cttgaaacgc
      421 tctcaatctt ggatatgata taaattctgg ataagatatg atggatatga tctatgtttg
      481 atagagtgca tttgaaagga taacggccct gcagacattt ttgaaaaaat aacaaacatt
      541 taattttctc actcgtttag gctattcccc tatttttgat attccttcgc acatatgaaa
      601 actacttttt ttcgaaactg ttaactccag aatttataaa tctatagccc ttacttgatt
      661 atttattatc atggttactc acctcatgtc tctttctctt ttcaagaagt cttcgttcat
      721 atctgttgtc tctttcctcc ttttgaatct ctgctttgct cttcgccatt gtttcctgaa
      781 aataatgtaa cttgaattgt gtaatatact tttttaattt gaatttggct tgtaacgcgg
      841 aatcactacg tgcgggatca tttcttacta gaaaacccag aaaatgccat atttcactta
      901 tctcggggtc atttctaatt agaaaagcta caaaaaccat ttctaattac ggtgacttta
      961 gaactggtcg aaatagctaa ttagaaatgg gtttttgcta attagaggtg actctatgtt
     1021 atctgagtaa gcctcgtttg tatgtaaact cacagtacta ataaatgcaa gacacccggg
     1081 tttgtctaga tatgaaataa ttgaaatatc aattctgaca gacaataatg gtaatcttga
     1141 taaggagttc cacgcccagg agaacacgtt agttttcttg tttttgattg cgtgcgttat
     1201 tttggagaaa aactcgattt tttacaaaat aattttttga aaggaacact gttcaataag
     1261 ttttgtcttt tttctcagtt gtgatacggt tttttattct tttttgtagt tatacagaag
     1321 accgttacga aacagactga tatcgaaaca aacactgatc ttacttgcac ttataatacg
     1381 actcactagt gggcagatct tcgaatgcat cgcgcgcacc gtacgtctcg agtgtaaaac
     1441 gacggccagt gaattatcaa ctatgtataa taaagttgaa tttcgtataa agaattcaat
     1501 caaataacca tgtattcaac agtattttgg atgcacatgt taggtgaaga gaaaaacata
     1561 attgaaaaaa cagaaactaa catttagtac atttttctct aaaaaagacg aaactcgcac
     1621 aaataaagga agaaaactgt tcaataacag ggcaaacgag atgagaagag accagtggca
     1681 cggataagga ttgaatttga aaaaggaata aaaatgatat tatgtaccac tttgtacaag
     1741 aaagctgggt ttacttgctg gaagtgtact tggtgacggc cttggttccc tcagacacgg
     1801 cgtgcttggc aagttctcct gggagaatca aacggacagc ggtttgaatt tcgcgggatg
     1861 agatcgttga gcgtttgttg taatgagcaa gacgggaagc ttccgaagcg atgcgttcga
     1921 atacatcgtt gacgaaggag ttcatgatag acatggcctt ggaggagact ccggtgtctg
     1981 ggtgaacttg cttgagaaca cggtagatgt agacggagta cgattccttg cgggcatgac
     2041 gtctcttctt tccgtccttt ggcttggcaa cgacggtctt ggcggccttc ttggctccct
     2101 tggcagatgg ctttggtgga ctagttctgg agcggccgcc accggtggag ctccaccgat
     2161 cccccgggca tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
     2221 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg
     2281 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
     2341 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttctgaa
     2401 aatttaaatc agattagtta gttagtaaaa tcatgtttaa acttacaact ttgattccat
     2461 tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt
     2521 gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa
     2581 gggtatcacc ttcaaacttg acttcagcac ctgaaaattt aaatatgtat ggttagttag
     2641 taccgaactg tttaaactta cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
     2701 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgccgt ttcatatgat
     2761 ctgggtatct cgagaagcat tgaacaccat aacagaaagt agtgacaagt gttggctgaa
     2821 aatttaaata atcagggtta gttagtatat atatgtttaa acttacccat ggaacaggta
     2881 gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca tcaccttcac
     2941 cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg
     3001 ggacaactcc agtgaaaagt tcttctcctc tagacacatt gatcctagca gaagcacagg
     3061 ctgcagggtg acggtccatc ccgctctcct gggcacaaga catgggcagc gtgccatcat
     3121 cctgcgccgc caccgccggc gggaagccat ggctaagtct agacatggag cctgcttttt
     3181 tgtacaaact tgtcattctc tgtctgaaac attcaattga ttatcgataa aattaattaa
     3241 gagagaatac aatgagaaaa cggaaaatcg agcaggattt tggggaaagt cggggaattt
     3301 ttgctattta agctttcttt aacaaagaaa ctgcattttt ccatgagatt accatgaaat
     3361 ttgcgttaaa tgggtgaatt tataacattt acggctagaa attaatttta aaaaagagaa
     3421 taaacgaaaa attattcgag aaaacgtctt tgaagcagaa atgaaatgaa aaaacaaccg
     3481 ccagcgctga caaaaatgat caaacaaacg gagtacggta tacgcggtaa gacccaaatg
     3541 aattttaatt cggtgcgcct ttaaaaaatg tattttttta atttctttat ccgtgggccc
     3601 aaatgtattt ttttcttgtg gtttgatgta aaaattgggt tatattattt gttttatggt
     3661 ttaatttttt aaaaactgat atcaactttt ctatacaaag ttgatagctt ggcgtaatca
     3721 tggtcatagc tgttcctgcc taggtgagct ctggtaccct ctagtcaagg ccttaagtga
     3781 gtcgtattac gtagcccggg cctagttcta gacattctct aatgaaaaaa tctttcagtt
     3841 gaaattgaaa atgagttaaa gttggagttt ttattgaaaa cagatttccg tgtgattagt
     3901 gtttttagcg agtgtgacag gacagcgaaa aaatatagaa acaagggggg aactgaaaag
     3961 cttaggaatg cattgaacat gagaagggga aggggaagga acaaactaga caggaattat
     4021 tggaatttaa tcacatttgg agtttttttt ctattcgaca gaataattat ccagaacatt
     4081 tttgtattaa atatttatgc atcatatgag tagtcggctt tgttgtgcat gacgagtttg
     4141 ttatcgacga aatagaagct gtcagaacga gtctcgtttg gattgttgat catgtcgtcc
     4201 actgaaaaag agattagtct tttgaattgt actttttaga ataatgactc actgagttgt
     4261 tgagagagtt gagggaactc atagatatgt tcacagttgt ttcgtgaatt cggaatacag
     4321 aatccgaatt caaagtcaaa acacttcaga aggcgatctt tgaagaagtg acgttcgatc
     4381 attcggaaat gatggattgg gatgtctcct actttgaatt ccacagttgc tccgaccgtt
     4441 ttgagtttca aaaagtttgg agcaaatcta taacgcacgt atcttgccga ctcctgtggc
     4501 gactcatcat tctcctgatc gttctccggt ttggcgatct cgaaaagcac ttgctcagtg
     4561 tccagatcac ggatttggaa cttggtgaac tcgatgttat agatgttcgc agatggggag
     4621 cataagaatc ctaaatttat gttttaaact gaaatccaaa gggagcaaga taccttgagt
     4681 gattcccgga agtgctaaaa cgtcgttcgg agtgatttga gctttcttcg caagctccga
     4741 ttccgttgtg attccttgtt cggtgcttgg tggtggccgt ggcatctgga aatatggaaa
     4801 agttcaacaa aaagaaaaga gaaaagaatg aaatcggata tcaagagtta gttgagcggt
     4861 ttctctagtt ttctgagtct cacctgcgac gggaaggtcg ccgagccggg tggaatcgat
     4921 cgttgttgct cggctttcat atcggtttgg ttggaagcgg ctgaaaacgg aaagaagtgg
     4981 aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt agagggtgcc aaataaccag
     5041 ctatattttg tttttttttg aaaacatttt taaaaagaaa aatacgataa tgatatcaga
     5101 tggatttccg gaaaactggt atgaaaaatt tcaacctttt tgagtacatg taatcaaaat
     5161 acactttgta aattatcatt tttattgaaa ctccaccatt tttctattta taacgctaat
     5221 aatttgaaaa agaaacctat tgcgaaccgc ggggtgaatc ccaaaaacga atgcgttttg
     5281 gtggagtgat tgattcgaat cgaagaagaa aaagaagaag acgtggaata gagagctcac
     5341 tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa atgaatgagg gtcttcttct
     5401 tcttcttctt cgaatgattg acagaaatgg gaaaaagagg aagattgaga agggaaaaag
     5461 gaaggagaaa agaagcagaa gaagacgtca gagaggagag gaacgagcgg aaaagcagcg
     5521 ggcgcaagtc atagaagtag cagagctggg gagaagaaga cactatccaa gaaaggaatg
     5581 acgagagagt atgcaaaggg gtatagggtg cagacagaat aggaacagaa taacagatga
     5641 tgagccaaga agagttgaaa agggcgatga atttgtcatg taacttaatt tgggtcaatt
     5701 tgagcatgat gaattgaaat catcccttgt tgggagttaa taaccggttt gttatcagaa
     5761 accctgtaat agaagggcgc cctaactttg agccaattca tcccggtttc tgtcaaatat
     5821 atcaaaaagt ggtcaactga caaattgttt ttgatattat aataaacatt ttatccgtta
     5881 acaattttcg aatacttttt acaaggactt ggataaattg gctcaaagac gaagtgagtt
     5941 tgctaccatc atggaatcag gaggacattt cgacaatgtc atataatgtt tgaaaaatga
     6001 ttttaacaag ggtctcattc gagataagca atgctctgag caagtggctc gtcgtcttca
     6061 agaatctctt gttgatattc atttggatcc tgttttgcac gaagcatgtg ccatggatat
     6121 tcagagtttt tgcaaaaaag ttccgccagg tcaaacaatc tttttaattt ttatatgata
     6181 cttcgggtgt atttttcttc tttttcctgt tcattttcgc ttttttctca cttttttgta
     6241 acaaaatctc tcacttttct gtaagtctta acaaaagttc aattggctcg atcaagtaaa
     6301 atttcggccg tatgaacgtt ttcaaaggtt ttcatttttt tttaagttca ttcacaatat
     6361 catgaatctc tcgtcctttg gtttccggta agtagcgaaa taatataagc atacttataa
     6421 atgaaggaat tgaatataga ataaggaaag cataactacc aataacggaa tatagaggta
     6481 atactgtaaa tgtactgata accaccatta cagtattcat tgaatatgcc acagattgag
     6541 caatactacg atgtttttga ggtaccagtt cagaggatat aaaccacgaa attggaccaa
     6601 ctccagttct gaaatttcat tattcgatta aatcaaaata tatttgaaaa acctcaccca
     6661 taagtataac cgtagatgat caatgcaact aggcatccgt atttcatcgg gtcaatcaga
     6721 atacgtattt ttgcgaagaa tacgaatgca attagagcta aattattgag aaatgtgaag
     6781 agaaggagaa gcttgcgtcg accaactctg taaacttatt catacaattt tgtcttcaaa
     6841 ttttgaacgt ttaaaactat cttattcaat gagttagcgg gtttttatag tgagttagtt
     6901 cacgattatc taataaaact atgtccaaac atcctaactg cgaccgtatt ctcactaact
     6961 cgtcttaaaa acgttgagat tattttaaac ccttcttcag tacaactaca gtactgatgc
     7021 acaaatacaa taataataat acattcagac atactctgaa caatgtaact tgtttttgtt
     7081 atactgtggt aacatttatt gaaatgaaat ataaccttac ttttcaatca caattccacc
     7141 agtaattgtt ccagaaacat aagctaaacc cattgcagtt gtactccatt cagcaatttc
     7201 tgactcaaca tttgcatttt ctagaaagaa agtagaggat aaaagaagag cccataatcc
     7261 gacagggccc tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg
     7321 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7381 accaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8281 ttaccatctg accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8461 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8761 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9181 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9241 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9301 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9361 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat
     9421 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     9481 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     9541 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     9601 ccagtcacga cgtcacagca cgtgtgtttt tgaacacggc gatatgtatc tgtagatcat
     9661 ccccaatttt caacaatga
//
