LOCUS       sequence-162398-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-ets4
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      493..1081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     982..1001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2717..2728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4794..4814
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5304..5328
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5490..5518)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6266..6286
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(6747..6771)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7535..7555)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7563..7580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7563..7579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9406..9428
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9466..9484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9552..9656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9657..9725,9726..10195,1..322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9875..9894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
       61 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      121 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      181 tcgcggtatc attgcagcac tggggtcaga tggtaagccc tcccgtatcg tagttatcta
      241 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      301 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
      361 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
      421 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      781 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      841 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      901 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      961 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1021 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1081 aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat
     1141 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1201 tgataccagc ggataacaat ttcacagggc cctgtcggat tatgggctct tcttttatcc
     1261 tctactttct ttctagaaaa tgcaaatgtt gagtcagaaa ttgctgaatg gagtacaact
     1321 gcaatgggtt tagcttatgt ttctggaaca attactggtg gaattgtgat tgaaaagtaa
     1381 ggttatattt catttcaata aatgttacca cagtataaca aaaacaagtt acattgttca
     1441 gagtatgtct gaatgtatta ttattattgt atttgtgcat cagtactgta gttgtactga
     1501 agaagggttt aaaataatct caacgttttt aagacgagtt agtgagaata cggtcgcagt
     1561 taggatgttt ggacatagtt ttattagata atcgtgaact aactcactat aaaaacccgc
     1621 taactcattg aataagatag ttttaaacgt tcaaaatttg aagacaaaat tgtatgaata
     1681 agtttacaga gttggtcgac gcaagcttct ccttctcttc acatttctca ataatttagc
     1741 tctaattgca ttcgtattct tcgcaaaaat acgtattctg attgacccga tgaaatacgg
     1801 atgcctagtt gcattgatca tctacggtta tacttatggg tgaggttttt caaatatatt
     1861 ttgatttaat cgaataatga aatttcagaa ctggagttgg tccaatttcg tggtttatat
     1921 cctctgaact ggtacctcaa aaacatcgta gtattgctca atctgtggca tattcaatga
     1981 atactgtaat ggtggttatc agtacattta cagtattacc tctatattcc gttattggta
     2041 gttatgcttt ccttattcta tattcaattc cttcatttat aagtatgctt atattatttc
     2101 gctacttacc ggaaaccaaa ggacgagaga ttcatgatat tgtgaatgaa cttaaaaaaa
     2161 aatgaaaacc tttgaaaacg ttcatacggc cgaaatttta cttgatcgag ccaattgaac
     2221 ttttgttaag acttacagaa aagtgagaga ttttgttaca aaaaagtgag aaaaaagcga
     2281 aaatgaacag gaaaaagaag aaaaatacac ccgaagtatc atataaaaat taaaaagatt
     2341 gtttgacctg gcggaacttt tttgcaaaaa ctctgaatat ccatggcaca tgcttcgtgc
     2401 aaaacaggat ccaaatgaat atcaacaaga gattcttgaa gacgacgagc cacttgctca
     2461 gagcattgct tatctcgaat gagacccttg ttaaaatcat ttttcaaaca ttatatgaca
     2521 ttgtcgaaat gtcctcctga ttccatgatg gtagcaaact cacttcgtct ttgagccaat
     2581 ttatccaagt ccttgtaaaa agtattcgaa aattgttaac ggataaaatg tttattataa
     2641 tatcaaaaac aatttgtcag ttgaccactt tttgatatat ttgacagaaa ccgggatgaa
     2701 ttggctcaaa gttagggcgc ccttctatta cagggtttct gataacaaac cggttattaa
     2761 ctcccaacaa gggatgattt caattcatca tgctcaaatt gacccaaatt aagttacatg
     2821 acaaattcat cgcccttttc aactcttctt ggctcatcat ctgttattct gttcctattc
     2881 tgtctgcacc ctatacccct ttgcatactc tctcgtcatt cctttcttgg atagtgtctt
     2941 cttctcccca gctctgctac ttctatgact tgcgcccgct gcttttccgc tcgttcctct
     3001 cctctctgac gtcttcttct gcttcttttc tccttccttt ttcccttctc aatcttcctc
     3061 tttttcccat ttctgtcaat cattcgaaga agaagaagaa gaagaccctc attcatttca
     3121 tttttttttc tgtcggtgtg tgctgctcgg ttaagagtga gctctctatt ccacgtcttc
     3181 ttctttttct tcttcgattc gaatcaatca ctccaccaaa acgcattcgt ttttgggatt
     3241 caccccgcgg ttcgcaatag gtttcttttt caaattatta gcgttataaa tagaaaaatg
     3301 gtggagtttc aataaaaatg ataatttaca aagtgtattt tgattacatg tactcaaaaa
     3361 ggttgaaatt tttcatacca gttttccgga aatccatctg atatcattat cgtatttttc
     3421 tttttaaaaa tgttttcaaa aaaaaacaaa atatagctgg ttatttggca ccctctaatt
     3481 accattttcc tgtcacacca cactcttttc cttcttccac ttctttccgt tttcagccgc
     3541 ttccaaccaa accgatatga aagccgagca acaacgatcg attccacccg gctcggcgac
     3601 cttcccgtcg caggtgagac tcagaaaact agagaaaccg ctcaactaac tcttgatatc
     3661 cgatttcatt cttttctctt ttctttttgt tgaacttttc catatttcca gatgccacgg
     3721 ccaccaccaa gcaccgaaca aggaatcaca acggaatcgg agcttgcgaa gaaagctcaa
     3781 atcactccga acgacgtttt agcacttccg ggaatcactc aaggtatctt gctccctttg
     3841 gatttcagtt taaaacataa atttaggatt cttatgctcc ccatctgcga acatctataa
     3901 catcgagttc accaagttcc aaatccgtga tctggacact gagcaagtgc ttttcgagat
     3961 cgccaaaccg gagaacgatc aggagaatga tgagtcgcca caggagtcgg caagatacgt
     4021 gcgttataga tttgctccaa actttttgaa actcaaaacg gtcggagcaa ctgtggaatt
     4081 caaagtagga gacatcccaa tccatcattt ccgaatgatc gaacgtcact tcttcaaaga
     4141 tcgccttctg aagtgttttg actttgaatt cggattctgt attccgaatt cacgaaacaa
     4201 ctgtgaacat atctatgagt tccctcaact ctctcaacaa ctcagtgagt cattattcta
     4261 aaaagtacaa ttcaaaagac taatctcttt ttcagtggac gacatgatca acaatccaaa
     4321 cgagactcgt tctgacagct tctatttcgt cgataacaaa ctcgtcatgc acaacaaagc
     4381 cgactactca tatgatgcat aaatatttaa tacaaaaatg ttctggataa ttattctgtc
     4441 gaatagaaaa aaaactccaa atgtgattaa attccaataa ttcctgtcta gtttgttcct
     4501 tccccttccc cttctcatgt tcaatgcatt cctaagcttt tcagttcccc ccttgtttct
     4561 atattttttc gctgtcctgt cacactcgct aaaaacacta atcacacgga aatctgtttt
     4621 caataaaaac tccaacttta actcattttc aatttcaact gaaagatttt ttcattagag
     4681 aatgtctaga actaggcccg ggctacgtaa tacgactcac ttaaggcctt gactagaggg
     4741 taccagagct cacctaggca ggaacagcta tgaccatgat tacgccaagc tatcaacttt
     4801 gtatagaaaa gttgatatca gtttttaaaa aattaaacca taaaacaaat aatataaccc
     4861 aatttttaca tcaaaccaca agaaaaaaat acatttgggc ccacggataa agaaattaaa
     4921 aaaatacatt ttttaaaggc gcaccgaatt aaaattcatt tgggtcttac cgcgtatacc
     4981 gtactccgtt tgtttgatca tttttgtcag cgctggcggt tgttttttca tttcatttct
     5041 gcttcaaaga cgttttctcg aataattttt cgtttattct cttttttaaa attaatttct
     5101 agccgtaaat gttataaatt cacccattta acgcaaattt catggtaatc tcatggaaaa
     5161 atgcagtttc tttgttaaag aaagcttaaa tagcaaaaat tccccgactt tccccaaaat
     5221 cctgctcgat tttccgtttt ctcattgtat tctctcttaa ttaattttat cgataatcaa
     5281 ttgaatgttt cagacagaga atgacaagtt tgtacaaaaa agcaggctcc atgtctagac
     5341 ttagccatgg cttcccgccg gcggtggcgg cgcaggatga tggcacgctg cccatgtctt
     5401 gtgcccagga gagcgggatg gaccgtcacc ctgcagcctg tgcttctgct aggatcaatg
     5461 tgtctagagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     5521 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     5581 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa
     5641 acatatatat actaactaac cctgattatt taaattttca gccaacactt gtcactactt
     5701 tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt
     5761 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     5821 ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
     5881 tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     5941 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     6001 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa
     6061 acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa attagacaca
     6121 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     6181 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     6241 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     6301 cacatggcat ggatgaacta tacaaatgcc cgggggatcg gtggagctcc accggtggcg
     6361 gccgctccag aactagtcca ccaaagccat ctgccaaggg agccaagaag gccgccaaga
     6421 ccgtcgttgc caagccaaag gacggaaaga agagacgtca tgcccgcaag gaatcgtact
     6481 ccgtctacat ctaccgtgtt ctcaagcaag ttcacccaga caccggagtc tcctccaagg
     6541 ccatgtctat catgaactcc ttcgtcaacg atgtattcga acgcatcgct tcggaagctt
     6601 cccgtcttgc tcattacaac aaacgctcaa cgatctcatc ccgcgaaatt caaaccgctg
     6661 tccgtttgat tctcccagga gaacttgcca agcacgccgt gtctgaggga accaaggccg
     6721 tcaccaagta cacttccagc aagtaaaccc agctttcttg tacaaagtgg tcatctggca
     6781 gaaagacaac gacgtgttgc tatctgtgta tgtgtcaata ctctgtttac atttttcaac
     6841 ttgtcctgtc ggtccagtgt ggtcccattt cccgatatct gtgattcaag atatacttcc
     6901 atgtactgtt cgttgtagaa catattccta ttgtgtccca tacttctttt taatacccga
     6961 gtcaatcatt caaggtttta ttcaacttct ttattttctt tttttttctc attctttttc
     7021 aattgttggg aacaatcaaa aagtattaca aatgttatta ccatttagag tgtgtagttc
     7081 agatgtctga atattgtttc actcgattta tttattgtaa tgcaatattt actggtttga
     7141 aaccgtgcaa tatgttgttc tctatttatt ctcgttctca tgtcagaact cttcctctct
     7201 cccaaatact ttaccaaata acggctcata tatccaccag tgacagaaaa gtaagcgttt
     7261 tactgaactg ttcccgattg tatttatacc aatatgatta tgttaattat aacaattata
     7321 atattgtatc aaaaaaatgt tttaatttta cattccattt atactccagc ctccttcccc
     7381 tgtttactag cctagttatc ccccaaagaa attgtattac aacaatgtgt agtcccagtg
     7441 tttttttata tcattgtgca attatttatt cagattgtat tatatattaa tgaaccagat
     7501 aaatattttt tcaaaatata aaacttggct tttacaactt tattatacat agttgataat
     7561 tcactggccg tcgttttaca ctcgagacgt acggtgcgcg cgatgcattc gaagatctgc
     7621 ccactagtga gtcgtattat aagtgcaagt aagatcagtg tttgtttcga tatcagtctg
     7681 tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc acaactgaga
     7741 aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag
     7801 tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg
     7861 tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc aattatttca
     7921 tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca tacaaacgag
     7981 gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct aattagctat
     8041 ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt ctaattagaa
     8101 atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga aatgatcccg
     8161 cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt acacaattca
     8221 agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa aaggaggaaa
     8281 gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag gtgagtaacc
     8341 atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt aacagtttcg
     8401 aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata gcctaaacga
     8461 gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt tatcctttca
     8521 aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt tatatcatat
     8581 ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg tagatcgaca
     8641 acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca agtttgtatt
     8701 tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg ccagatcgat
     8761 gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg gccacggatt
     8821 gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac aaaatatggt
     8881 tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag atattttggg
     8941 tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac acaaaaagtt
     9001 cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat cgccgtgttc
     9061 aaaaacacac gtgctgtgac gtcgtgactg gggaaaaccc tggcgttacc caacttaatc
     9121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9181 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     9241 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtgca atctgctctg
     9301 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9361 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9421 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     9481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgat
//
