LOCUS       sequence-162400-        4014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BMP4 , used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_BMP4_WT_V5
FEATURES             Location/Qualifiers
     source          1..4014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..90
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     324..341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(500..527)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(619..705)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     754..776
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     768..785
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     769..785
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    801..900
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2125..2224)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2241..2260)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2242..2260)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2265..2281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2265..2281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2712..3503
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3596..4014,1..170)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
       61 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      121 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      181 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      241 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      301 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      421 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
      481 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
      541 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
      601 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      661 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
      721 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
      781 ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
      841 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca
      901 tgattcctgg taaccgaatg ctgatggtcg ttttattatg ccaagtcctg ctaggaggcg
      961 cgagccatgc tagtttgata cctgagacgg ggaagaaaaa agtcgccgag attcagggcc
     1021 acgcgggagg acgccgctca gggcagagcc atgagctcct gcgggacttc gaggcgacac
     1081 ttctgcagat gtttgggctg cgccgccgcc cgcagcctag caagagtgcc gtcattccgg
     1141 actacatgcg ggatctttac cggcttcagt ctggggagga ggaggaagag cagatccaca
     1201 gcactggtct tgagtatcct gagcgcccgg ccagccgggc caacaccgtg aggagcttcc
     1261 accacgaaga acatctggag aacatcccag ggaccagtga aaactctgct tttcgtttcc
     1321 tctttaacct cagcagcatc cctgagaacg aggcgatctc ctctgcagag cttcggctct
     1381 tccgggagca ggtggaccag ggccctgatt gggaaagggg cttccaccgt ataaacattt
     1441 atgaggttat gaagccccca gcagaagtgg tgcctgggca cctcatcaca cgactactgg
     1501 acacgagact ggtccaccac aatgtgacac ggtgggaaac ttttgatgtg agccctgcgg
     1561 tccttcgctg gacccgggag aagcagccaa actatgggct agccattgag gtgactcacc
     1621 tccatcagac tcggacccac cagggccagc atgtcaggat tagccgatcg ttacctcaag
     1681 ggagtgggaa ttgggcccag ctccggcccc tcctggtcac ctttggccat gatggccggg
     1741 gccatgcctt gacccgacgc cggagggcca agcgtagccc taagcatcac tcacagcggg
     1801 ccaggaagaa gaataagaac tgccggcgcc actcgctcta tgtggacttc agcgatgtgg
     1861 gctggaatga ctggattgtg gccccaccag gctaccaggc cttctactgc catggggact
     1921 gcccctttcc actggctgac cacctcaact caaccaacca tgccattgtg cagaccctgg
     1981 tcaattctgt caattccagt atccccaaag cctgttgtgt gcccactgaa ctgagtgcca
     2041 tctccatgct gtacctggat gagtatgata aggtggtact gaaaaattat caggagatgg
     2101 tagtagaggg atgtgggtgc cgctgcccaa ctttcttgta caaagttggc attataagaa
     2161 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2221 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2281 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2341 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2401 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2461 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2521 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2581 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2641 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2701 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2761 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2821 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2881 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2941 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3001 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3061 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3121 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3181 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3241 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3301 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3361 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3421 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3481 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3541 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3601 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3661 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3721 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3781 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3841 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3901 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3961 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaag
//
