LOCUS       sequence-162401-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human
            GAPDH locus using GAPTrap vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-TALEN1
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtcgtgggtt tgtttaactt ggtaccataa ctagttcggc gcgccactag cgctgctcac
       61 gcgccaagcc gccaccatgg gcgatcctaa aaagaaacgt aaggtcatcg attacccata
      121 cgatgttcca gattacgcta tcgatatcgc cgatctacgc acgctcggct acagccagca
      181 gcaacaggag aagatcaaac cgaaggttcg ttcgacagtg gcgcagcacc acgaggcact
      241 ggtcggccac gggtttacac acgcgcacat cgttgcgtta agccaacacc cggcagcgtt
      301 agggaccgtc gctgtcaagt atcaggacat gatcgcagcg ttgccagagg cgacacacga
      361 agcgatcgtt ggcgtcggca aacagtggtc cggcgcacgc gctctggagg ccttgctcac
      421 ggtggcggga gagttgagag gtccaccgtt acagttggac acaggccaac ttctcaagat
      481 tgcaaaacgt ggcggcgtga ccgcagtgga ggcagtgcat gcatggcgca atgcactgac
      541 gggtgccccg ctcaacttga ccccggagca ggtggtggcc atcgccagcc acgatggcgg
      601 caagcaggcg ctggagacgg tccagcggct gttgccggtg ctgtgccagg cccacggctt
      661 gaccccggag caggtggtgg ccatcgccag ccacgatggc ggcaagcagg cgctggagac
      721 ggtccagcgg ctgttgccgg tgctgtgcca ggcccacggc ttgaccccgg agcaggtggt
      781 ggccatcgcc agcaatattg gtggcaagca ggcgctggag acggtgcagg cgctgttgcc
      841 ggtgctgtgc ccaggcccac ggcttgaccc cggagcaggt ggtggccatc gccagcaata
      901 ttggtggcaa gcaggcgctg gagacggtgc aggcgctgtt gccggtgctg tgccaggccc
      961 acggcttgac ccc
//
