LOCUS       sequence-162404-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human
            GAPDH locus using GAPTrap vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap TALEN 2
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             109..153
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtgggtttgt ttaacttggt accataacta gttcggcgcg ccactagcgc tgtcacgcgc
       61 caagccgcca ccatgggcga tcctaaaaag aaacgtaagg tcatcgataa ggagaccgcc
      121 gctgccaagt tcgagagaca gcacatggac agcatcgata tcgccgatct acgcacgctc
      181 ggctacagcc agcagcaaca ggagaagatc aaaccgaagg ttcgttcgac agtggcgcag
      241 caccacgagg cactggtcgg ccacgggttt acacacgcgc acatcgttgc gttaagccaa
      301 cacccggcag cgttagggac cgtcgctgtc aagtatcagg acatgatcgc agcgttgcca
      361 gaggcgacac acgaagcgat cgttggcgtc ggcaaacagt ggtccggcgc acgcgctctg
      421 gaggccttgc tcacggtggc gggagagttg agaggtccac cgttacagtt ggacacaggc
      481 caacttctca agattgcaaa acgtggcggc gtgaccgcag tggaggcagt gcatgcatgg
      541 cgcaatgcac tgacgggtgc cccgctcaac ttgaccccgg agcaggtggt ggccatcgcc
      601 agccacgatg gcggcaagca ggcgctggag acggtccagc ggctgttgcc ggtgctgtgc
      661 caggcccacg gcttgacccc ccagcaggtg gtggccatcg ccagcaatgg cggtggcaag
      721 caggcgctgg agacggtcca gcggctgttg ccggtgctgt gccaggccca cggcttgacc
      781 ccggagcagg tggtggccat cgccagccac gatggcggca agcaggcgct ggagacggtc
      841 cagcggctgt tgccggtgct gtgccaggcc cacggcttga ccccccagca ggtggtggcc
      901 atcgccagca atggcggtgg caagcaggcg ctggagacgg tccagcggct gttgccggtg
      961 ctgtgccagg cccacggctt gaccccccag caggtggtgg ccatcgccag caatggcggt
     1021 ggcaa
//
