LOCUS       sequence-162411-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at yeast URA3 locus, expression 
            from the GPD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Nemhauser plasmids
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGP6G-ccdB
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(267..283)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(268..284)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      286..532
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(302..320)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatggg
      241 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagtcatg taattagtta
      301 tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta
      361 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
      421 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
      481 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgatgtcgta
      541 ataaccccgc cccgaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
      601 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttctata
      661 atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat
      721 ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag
      781 acaggactgt aaagatggac gcattgaact ccaaagaaca acaagagttc caaaaagtag
      841 tggaacaaa
//
