LOCUS       sequence-162416-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at HIS3, expression from the GPD 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Nemhauser plasmids
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGP8G-ccdB
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..73
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     57..73
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    81..205
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(230..235,236..253,254..260)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(380..399)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttttatagtt agtctttttt ttagttttaa aacaccagaa cttagtttcg acggattcta
       61 gaactagtgg atcccccatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
      121 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
      181 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
      301 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
      361 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
      421 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
      481 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
      541 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
      601 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
      661 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
      721 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
      781 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
      841 ttatacgca
//
