LOCUS       sequence-162418-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-HygMx4
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..383
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgcaagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
       61 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatc
      121 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
      181 cgaagcaaaa attacggccc ctcgctgcgg acctgcgagc agggaaacgc tcccctcaca
      241 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
      301 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
      361 atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg acgtctgtcg
      421 agaagtttct gatcgaacag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
      481 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
      541 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
      601 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
      661 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
      721 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
      781 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
      841 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
      901 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cc
//
