LOCUS       sequence-162421-        3546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HSD17B10, used as an over expression
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_HSD17B10_WT_V5
FEATURES             Location/Qualifiers
     source          1..3546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(57..156)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(173..192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(174..192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(197..213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(197..213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             644..1435
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1528..2116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2017..2036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2270..2287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2446..2473)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2565..2651)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2700..2722
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2714..2731
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2715..2731
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2747..2846
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccattcctc aatggagagg tcatccggct ggatggggcc attcgtatgc agccttgccc
       61 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      121 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
      181 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
      241 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
      301 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
      361 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
      421 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
      481 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
      541 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
      601 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
      661 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
      721 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
      781 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
      841 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
      901 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
      961 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1021 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1081 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1141 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1201 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1261 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1321 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1381 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     1441 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     1501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2161 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2221 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2281 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2341 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     2401 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     2461 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     2521 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     2581 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     2641 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
     2701 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
     2761 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
     2821 gccaactttg tacaaaaaag ttggcatggc agcagcgtgt cggagcgtga agggcctggt
     2881 ggcggtaata accggaggag cctcgggcct gggcctggcc acggcggagc gacttgtggg
     2941 gcagggagcc tctgctgtgc ttctggacct gcccaactcg ggtggggagg cccaagccaa
     3001 gaagttagga aacaactgcg ttttcgcccc agccgacgtg acctctgaga aggatgtgca
     3061 aacagctctg gctctagcaa aaggaaagtt tggccgtgtg gatgtagctg tcaactgtgc
     3121 aggcatcgcg gtggctagca agacgtacaa cttaaagaag ggccagaccc ataccttgga
     3181 agacttccag cgagttcttg atgtgaatct catgggcacc ttcaatgtga tccgcctggt
     3241 ggctggtgag atgggccaga atgaaccaga ccagggaggc caacgtgggg tcatcatcaa
     3301 cactgccagt gtggctgcct tcgagggtca ggttggacaa gctgcatact ctgcttccaa
     3361 ggggggaata gtgggcatga cactgcccat tgctcgggat ctggctccca taggtctgtt
     3421 tggcacccca ctgctgacca gcctcccaga gaaagtgtgc aacttcttgg ccagccaagt
     3481 gcccttccct agccgactgg gtgaccctgc tgagtatgct cacctcgtac aggccatcat
     3541 cgagaa
//
