LOCUS       sequence-162423-        5244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK7, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MAPK7_p.D46N
FEATURES             Location/Qualifiers
     source          1..5244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2644..2743)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2760..2779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2761..2779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2784..2800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2784..2800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3231..4022
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4115..4703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4604..4623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4857..4874
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5033..5060)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5152..5238)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggccgag cctctgaagg aggaagacgg cgaggacggc tctgcggagc
      241 cccccgggcc cgtgaaggcc gaacccgccc acaccgctgc ctctgtagcg gccaagaacc
      301 tggccctgct taaagcccgc tccttcaatg tgacctttga cgtgggcgac gagtacgaga
      361 tcatcgagac cataggcaac ggggcctatg gagtggtgtc ctccgcccgc cgccgcctca
      421 ccggccagca ggtggccatc aagaagatcc ctaatgcttt cgatgtggtg accaatgcca
      481 agcggaccct cagggagctg aagatcctca agcactttaa acacgacaac atcatcgcca
      541 tcaaggacat cctgaggccc accgtgccct atggcgaatt caaatctgtc tacgtggtcc
      601 tggacctgat ggaaagcgac ctgcaccaga tcatccactc ctcacagccc ctcacactgg
      661 aacacgtgcg ctacttcctg taccaactgc tgcggggcct gaagtacatg cactcggctc
      721 aggtcatcca ccgtgacctg aagccctcca acctattggt gaatgagaac tgtgagctca
      781 agattggtga ctttggtatg gctcgtggcc tgtgcacctc gcccgctgaa catcagtact
      841 tcatgactga gtatgtggcc acgcgctggt accgtgcgcc cgagctcatg ctctctttgc
      901 atgagtatac acaggctatt gacctctggt ctgtgggctg catctttggt gagatgctgg
      961 cccggcgcca gctcttccca ggcaaaaact atgtacacca gctacagctc atcatgatgg
     1021 tgctgggtac cccatcacca gccgtgattc aggctgtggg ggctgagagg gtgcgggcct
     1081 atatccagag cttgccacca cgccagcctg tgccctggga gacagtgtac ccaggtgccg
     1141 accgccaggc cctatcactg ctgggtcgca tgctgcgttt tgagcccagc gctcgcatct
     1201 cagcagctgc tgcccttcgc caccctttcc tggccaagta ccatgatcct gatgatgagc
     1261 ctgactgtgc cccgcccttt gactttgcct ttgaccgcga agccctcact cgggagcgca
     1321 ttaaggaggc cattgtggct gaaattgagg acttccatgc aaggcgtgag ggcatccgcc
     1381 aacagatccg cttccagcct tctctacagc ctgtggctag tgagcctggc tgtccagatg
     1441 ttgaaatgcc cagtccctgg gctcccagtg gggactgtgc catggagtct ccaccaccag
     1501 ccccgccacc atgccccggc cctgcacctg acaccattga tctgaccctg cagccacctc
     1561 caccagtcag tgagcctgcc ccaccaaaga aagatggtgc catctcagac aatactaagg
     1621 ctgcccttaa agctgccctg ctcaagtctt tgaggagccg gctcagagat ggccccagcg
     1681 cacccctgga ggctcctgag cctcggaagc cggtgacagc ccaggagcgc cagcgggagc
     1741 gggaggagaa gcggcggagg cggcaagaac gagccaagga gcgggagaaa cggcggcagg
     1801 agcgggagcg aaaggaacgg ggggctgggg cctctggggg cccctccact gaccccttgg
     1861 ctggactagt gctcagtgac aatgacagaa gcctgttgga acgctggact cgaatggccc
     1921 ggcccgcagc cccagccctc acctctgtgc cggcccctgc cccagcgcca acgccaaccc
     1981 caaccccagt ccaacctacc agtcctcctc ctggccctgt agcccagccc actggcccgc
     2041 aaccacaatc tgcgggctct acctctggcc ctgtacccca gcctgcctgc ccaccccctg
     2101 gccctgcacc ccaccccact ggccctcctg ggcccatccc tgtccccgcg ccaccccaga
     2161 ttgccacctc caccagcctc ctggctgccc agtcacttgt gccaccccct gggctgcctg
     2221 gctccagcac cccaggagtt ttgccttact tcccacctgg cctgccgccc ccagacgccg
     2281 ggggagcccc tcagtcttcc atgtcagagt cacctgatgt caaccttgtg acccagcagc
     2341 tatctaagtc acaggtggag gaccccctgc cccctgtgtt ctcaggcaca ccaaagggca
     2401 gtggggctgg ctacggtgtt ggctttgacc tggaggaatt cttaaaccag tctttcgaca
     2461 tgggcgtggc tgatgggcca caggatggcc aggcagattc agcctctctc tcagcctccc
     2521 tgcttgctga ctggctcgaa ggccatggca tgaaccctgc cgatattgag tccctgcagc
     2581 gtgagatcca gatggactcc ccaatgctgc tggctgacct gcctgacctc caggacccct
     2641 agttgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2701 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2761 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2821 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     2881 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     2941 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3001 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3061 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3121 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3181 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3241 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3301 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3361 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3421 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3481 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3541 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3601 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3661 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3721 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3781 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3841 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3901 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3961 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4021 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4261 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4801 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4861 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4921 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4981 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     5041 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5101 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5161 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5221 agcctttcgt tttatttgat gcct
//
