LOCUS       sequence-162424-       12630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti virus delivery of LbCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY109 (lenti-LbCpf1)
FEATURES             Location/Qualifiers
     source          1..12630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             72..119
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             126..206
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             216..272
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             273..869
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     766..786
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    885..1473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(938..958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1356..1367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1545..1778
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1804..1821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1810..2034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2080..2508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2167..2186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2377..2398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2517..2537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2522..2851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2702..2837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2764..2783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2899..2946
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2965..3339
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3469..3590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3506..3525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3560..3579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3639..3655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3639..3655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3652..3674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3663..3679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3687..3693,3694..3711,3712..3717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3732..3753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3870..3887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4041..4629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4121..4140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4800..5591,5592..5660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5423..5442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5661..5765)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5840..5859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6031..6410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6412..6610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6565..6585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6628..6808
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6855..6980
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7473..7706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7891..7935
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8233..8350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(8446..8694)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8504..8523)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8674..8694)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        8745..8956
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      8964..8973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8976..8996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9021..12630,1..71)
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
ORIGIN
        1 agctggataa ggtgaagatc gccatctcta acaaggagtg gctggagtac gcccagacca
       61 gcgtgaagca caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
      121 gatcctaccc atacgatgtt ccagattacg cttatcccta cgacgtgcct gattatgcat
      181 acccatatga tgtccccgac tatgccggat ccggcgcaac aaacttctct ctgctgaaac
      241 aagccggaga tgtcgaagag aatcctggac cgaccgagta caagcccacg gtgcgcctcg
      301 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
      361 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
      421 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
      481 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
      541 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
      601 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
      661 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      721 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      781 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
      841 ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt
      901 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
      961 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     1021 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     1081 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     1141 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     1201 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1261 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     1321 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     1381 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     1441 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     1501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     1561 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     1621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     1801 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1861 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1921 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1981 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2041 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     2101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     2521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2761 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     2821 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     2881 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     2941 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     3001 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     3061 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     3121 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     3181 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     3241 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     3301 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     3361 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     3421 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     3481 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3541 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     3601 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     3661 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3721 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3841 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4021 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4801 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4861 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4921 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4981 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5041 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5101 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5161 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5221 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5281 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5341 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5401 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5461 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5521 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5581 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5641 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5761 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     5821 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     5881 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     5941 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     6001 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     6061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     6121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     6241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     6421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6601 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6661 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6721 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6781 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6841 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6901 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     6961 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7021 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7081 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7141 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7201 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7261 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7321 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7381 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7441 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7501 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7561 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7621 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7681 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7741 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7801 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7861 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7921 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     7981 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8041 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8101 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     8161 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     8221 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8281 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8341 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa tttaattaaa
     8401 aaaagagacg atatatcgtc tccatctaca cttagtagaa attacggtgt ttcgtccttt
     8461 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
     8521 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
     8581 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct
     8641 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttctcg
     8701 agaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     8761 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag
     8821 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     8881 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     8941 tgccgccaga acacaggtcc ggtgccacca tggccccaaa gaagaagcgg aaggtcggta
     9001 tccacggagt cccagcagcc agcaagctgg agaagtttac aaactgctac tccctgtcta
     9061 agaccctgag gttcaaggcc atccctgtgg gcaagaccca ggagaacatc gacaataagc
     9121 ggctgctggt ggaggacgag aagagagccg aggattataa gggcgtgaag aagctgctgg
     9181 atcgctacta tctgtctttt atcaacgacg tgctgcacag catcaagctg aagaatctga
     9241 acaattacat cagcctgttc cggaagaaaa ccagaaccga gaaggagaat aaggagctgg
     9301 agaacctgga gatcaatctg cggaaggaga tcgccaaggc cttcaagggc aacgagggct
     9361 acaagtccct gtttaagaag gatatcatcg agacaatcct gccagagttc ctggacgata
     9421 aggacgagat cgccctggtg aacagcttca atggctttac cacagccttc accggcttct
     9481 ttgataacag agagaatatg ttttccgagg aggccaagag cacatccatc gccttcaggt
     9541 gtatcaacga gaatctgacc cgctacatct ctaatatgga catcttcgag aaggtggacg
     9601 ccatctttga taagcacgag gtgcaggaga tcaaggagaa gatcctgaac agcgactatg
     9661 atgtggagga tttctttgag ggcgagttct ttaactttgt gctgacacag gagggcatcg
     9721 acgtgtataa cgccatcatc ggcggcttcg tgaccgagag cggcgagaag atcaagggcc
     9781 tgaacgagta catcaacctg tataatcaga aaaccaagca gaagctgcct aagtttaagc
     9841 cactgtataa gcaggtgctg agcgatcggg agtctctgag cttctacggc gagggctata
     9901 catccgatga ggaggtgctg gaggtgttta gaaacaccct gaacaagaac agcgagatct
     9961 tcagctccat caagaagctg gagaagctgt tcaagaattt tgacgagtac tctagcgccg
    10021 gcatctttgt gaagaacggc cccgccatca gcacaatctc caaggatatc ttcggcgagt
    10081 ggaacgtgat ccgggacaag tggaatgccg agtatgacga tatccacctg aagaagaagg
    10141 ccgtggtgac cgagaagtac gaggacgatc ggagaaagtc cttcaagaag atcggctcct
    10201 tttctctgga gcagctgcag gagtacgccg acgccgatct gtctgtggtg gagaagctga
    10261 aggagatcat catccagaag gtggatgaga tctacaaggt gtatggctcc tctgagaagc
    10321 tgttcgacgc cgattttgtg ctggagaaga gcctgaagaa gaacgacgcc gtggtggcca
    10381 tcatgaagga cctgctggat tctgtgaaga gcttcgagaa ttacatcaag gccttctttg
    10441 gcgagggcaa ggagacaaac agggacgagt ccttctatgg cgattttgtg ctggcctacg
    10501 acatcctgct gaaggtggac cacatctacg atgccatccg caattatgtg acccagaagc
    10561 cctactctaa ggataagttc aagctgtatt ttcagaaccc tcagttcatg ggcggctggg
    10621 acaaggataa ggagacagac tatcgggcca ccatcctgag atacggctcc aagtactatc
    10681 tggccatcat ggataagaag tacgccaagt gcctgcagaa gatcgacaag gacgatgtga
    10741 acggcaatta cgagaagatc aactataagc tgctgcccgg ccctaataag atgctgccaa
    10801 aggtgttctt ttctaagaag tggatggcct actataaccc cagcgaggac atccagaaga
    10861 tctacaagaa tggcacattc aagaagggcg atatgtttaa cctgaatgac tgtcacaagc
    10921 tgatcgactt ctttaaggat agcatctccc ggtatccaaa gtggtccaat gcctacgatt
    10981 tcaacttttc tgagacagag aagtataagg acatcgccgg cttttacaga gaggtggagg
    11041 agcagggcta taaggtgagc ttcgagtctg ccagcaagaa ggaggtggat aagctggtgg
    11101 aggagggcaa gctgtatatg ttccagatct ataacaagga cttttccgat aagtctcacg
    11161 gcacacccaa tctgcacacc atgtacttca agctgctgtt tgacgagaac aatcacggac
    11221 agatcaggct gagcggagga gcagagctgt tcatgaggcg cgcctccctg aagaaggagg
    11281 agctggtggt gcacccagcc aactccccta tcgccaacaa gaatccagat aatcccaaga
    11341 aaaccacaac cctgtcctac gacgtgtata aggataagag gttttctgag gaccagtacg
    11401 agctgcacat cccaatcgcc atcaataagt gccccaagaa catcttcaag atcaatacag
    11461 aggtgcgcgt gctgctgaag cacgacgata acccctatgt gatcggcatc gataggggcg
    11521 agcgcaatct gctgtatatc gtggtggtgg acggcaaggg caacatcgtg gagcagtatt
    11581 ccctgaacga gatcatcaac aacttcaacg gcatcaggat caagacagat taccactctc
    11641 tgctggacaa gaaggagaag gagaggttcg aggcccgcca gaactggacc tccatcgaga
    11701 atatcaagga gctgaaggcc ggctatatct ctcaggtggt gcacaagatc tgcgagctgg
    11761 tggagaagta cgatgccgtg atcgccctgg aggacctgaa ctctggcttt aagaatagcc
    11821 gcgtgaaggt ggagaagcag gtgtatcaga agttcgagaa gatgctgatc gataagctga
    11881 actacatggt ggacaagaag tctaatcctt gtgcaacagg cggcgccctg aagggctatc
    11941 agatcaccaa taagttcgag agctttaagt ccatgtctac ccagaacggc ttcatctttt
    12001 acatccctgc ctggctgaca tccaagatcg atccatctac cggctttgtg aacctgctga
    12061 aaaccaagta taccagcatc gccgattcca agaagttcat cagctccttt gacaggatca
    12121 tgtacgtgcc cgaggaggat ctgttcgagt ttgccctgga ctataagaac ttctctcgca
    12181 cagacgccga ttacatcaag aagtggaagc tgtactccta cggcaaccgg atcagaatct
    12241 tccggaatcc taagaagaac aacgtgttcg actgggagga ggtgtgcctg accagcgcct
    12301 ataaggagct gttcaacaag tacggcatca attatcagca gggcgatatc agagccctgc
    12361 tgtgcgagca gtccgacaag gccttctact ctagctttat ggccctgatg agcctgatgc
    12421 tgcagatgcg gaacagcatc acaggccgca ccgacgtgga ttttctgatc agccctgtga
    12481 agaactccga cggcatcttc tacgatagcc ggaactatga ggcccaggag aatgccatcc
    12541 tgccaaagaa cgccgacgcc aatggcgcct ataacatcgc cagaaaggtg ctgtgggcca
    12601 tcggccagtt caagaaggcc gaggacgaga
//
