LOCUS       sequence-162433-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses huLbCpf1-P2A-puro and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY31
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        170..418
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     170..190
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     341..360
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        539..918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        919..1122
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1073..1093
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1167..1186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1167..1185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1233..4916
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4917..4964
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4974..5030
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5031..5627
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5524..5544
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5702..5719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5708..5932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5978..6406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6065..6084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6275..6296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6415..6435)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6420..6749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6600..6735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6662..6681
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6816..7610
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6870..6889)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7480..7499
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7784..7905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7821..7840)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7875..7894
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7954..7970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7954..7970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7967..7989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8002..8008,8009..8026,8027..8032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8047..8068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8185..8202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8356..8941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8436..8455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9112..9903,9904..9972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9735..9754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9973..10077)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttaattaag agggcctatt
      181 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      241 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      301 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      361 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt
      421 aatttctact aagtgtagat ggagacgata tatcgtctct ttttcaattg catgaagaat
      481 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      541 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      601 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      661 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      721 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      781 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      841 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      901 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      961 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1021 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1081 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     1141 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1201 tagcgtttaa acttaagctt ggtaccgcca ccatgagcaa gctggagaag tttacaaact
     1261 gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga
     1321 acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg
     1381 tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca
     1441 agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg
     1501 agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca
     1561 agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag
     1621 agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag
     1681 ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat
     1741 ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct
     1801 tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc
     1861 tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga
     1921 cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg
     1981 agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc
     2041 tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct
     2101 acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca
     2161 agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg
     2221 agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg
     2281 atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc
     2341 acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca
     2401 agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg
     2461 tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg
     2521 gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg
     2581 acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca
     2641 tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt
     2701 ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt
     2761 atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt
     2821 tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg
     2881 gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg
     2941 acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta
     3001 ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg
     3061 aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga
     3121 atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt
     3181 ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt
     3241 acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg
     3301 tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt
     3361 ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg
     3421 agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct
     3481 ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc
     3541 cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt
     3601 ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct
     3661 tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg
     3721 gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca
     3781 tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga
     3841 cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact
     3901 ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca
     3961 agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg
     4021 gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc
     4081 tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg
     4141 ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga
     4201 acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct
     4261 ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct
     4321 cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata
     4381 agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca
     4441 accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt
     4501 gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg
     4561 atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc
     4621 tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc
     4681 tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc
     4741 aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa
     4801 aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga
     4861 agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa
     4921 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc ggcgcaacaa
     4981 acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg accgagtaca
     5041 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5101 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5161 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5221 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5281 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5341 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5401 tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5461 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5521 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5581 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaagta agaattctgc
     5641 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     5701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5881 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5941 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6661 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6721 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6781 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6961 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7321 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7621 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7681 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7741 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7921 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8761 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8881 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8941 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9001 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9061 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9121 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9181 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9241 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9301 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9361 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9421 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9481 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9541 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9601 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9661 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9721 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9781 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9841 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9901 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9961 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10021 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10081 atttccccga aaagtgccac ctgacgtc
//
