LOCUS       sequence-162448-        5583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM10 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM10_p.Y580F
FEATURES             Location/Qualifiers
     source          1..5583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(926..943)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(1542..1553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2983..3082)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3099..3118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3100..3118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3123..3139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3123..3139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3570..4361
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4454..5042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4943..4962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5196..5213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5372..5399)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5491..5577)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggagtatgaa agacgtggtg gtcgtggtga caggactggc cgctatggag
      241 ccactgaccg ctcgcaggat gatggtgggg agaaccgcag ccgagaccac gactaccggg
      301 acatggacta ccgttcatat cctcgcgagt atggcagcca ggagggcaag catgactatg
      361 acgactcatc tgaggagcag agtgcggagg attcctacga ggcctccccg ggctccgaga
      421 ctcagcgtag gcggcggcgg cggcacaggc acagccccac cggcccgcca ggcttccccc
      481 gagacggcga ctatcgggac caggactatc ggaccgagca aggggaggag gaggaggagg
      541 aggaggatga ggaggaggag gagaaggcca gtaacatcgt catgctgagg atgctgccac
      601 aggcagccac tgaggatgac atccgtggcc agctgcagtc gcacggcgtg caagcacggg
      661 aggttcggct gatgcggaac aaatcttcag gtcagagccg gggcttcgcc ttcgtcgagt
      721 ttagtcactt gcaggacgct acacgatgga tggaagccaa tcagcactcc ctcaacatcc
      781 tgggccagaa ggtgtcgatg cactacagtg accccaagcc caagatcaat gaggactggc
      841 tgtgcaataa gtgtggcgtc cagaacttca aacgccgaga gaagtgcttc aaatgtggcg
      901 tgcccaagtc agaggcagag cagaagctgc ccctcggcac gaggctggat cagcagacac
      961 tgccactggg tggccgggag ctgagccagg gcctgcttcc cctgccgcag ccctaccagg
     1021 cccagggagt cctggcctcc caagccctgt cacagggctc ggagccaagc tcagagaacg
     1081 ccaatgacac catcattttg cgcaacctga acccacacag caccatggat tccatcctgg
     1141 gggccctggc accctacgcg gtgctgtcct cctccaacgt gcgcgtcata aaggacaagc
     1201 agacccaact gaaccgcggc tttgccttca tccagctctc caccatcgtg gaggcagccc
     1261 agctgctgca gatcctgcag gccctgcacc caccactcac tatcgacggc aagaccatca
     1321 atgttgagtt tgccaagggt tctaagaggg acatggcctc caatgaaggc agtcgcatca
     1381 gtgctgcctc tgtggccagc actgccattg ctgcggccca gtgggccatc tcacaggcct
     1441 cccaaggtgg ggagggtacc tgggccacct ccgaggagcc gccggtcgac tacagctact
     1501 accaacagga tgagggctat ggcaacagcc agggcacaga gtcttccctc tatgcccatg
     1561 gctacctcaa gggcaccaag ggccctggca tcactggaac caaaggggat cccactggag
     1621 caggtcccga ggcctcccta gagcctgggg ccgactctgt gtcgatgcag gctttctctc
     1681 gcgcccagcc tggtgctgct cctggcatct accaacaatc agccgaggcg agcagtagcc
     1741 agggcactgc tgccaacagc cagtcgtata ccatcatgtc acccgctgtg ctcaaatctg
     1801 agctccagag ccctacccat cctagttctg ctctcccacc ggctaccagc cccactgccc
     1861 aggaatccta cagccagtac cctgttcccg acgtctctac ctaccagtac gatgagacct
     1921 ccggcttcta ctatgacccc cagaccggcc tctactatga ccccaactcc cagtattact
     1981 acaatgctca gagccagcag tacctgtact gggatgggga gaggcggacc tatgttcccg
     2041 ccctggagca gtcggccgac ggacataagg agacaggggc accctcgaag gagggcaaag
     2101 agaagaagga gaagcacaag accaagacag ctcaacagat tgccaaggac atggaacgct
     2161 gggcccgcag tctcaacaaa caaaaagaaa acttcaaaaa tagcttccag cctatcagct
     2221 ccctgcgaga tgacgagagg cgggagtcag ccactgcaga tgctggctat gccatcctcg
     2281 agaagaaggg agcactagcc gagagacagc acaccagcat ggatctcccg aaattggcca
     2341 gtgacgaccg cccaagccct ccgcgaggac tggtggcagc ctacagcggg gagagtgaca
     2401 gtgaggagga gcaggagcgt gggggccctg agcgggagga gaagctcacc gactggcaga
     2461 agctggcctg tctgctctgc cgacgccagt tccccagcaa agaggcgctc atccggcacc
     2521 agcagctctc agggctccac aagcaaaacc ttgagattca ccggcgagcc cacttgtcag
     2581 aaaacgagct agaagcacta gagaagaatg acatggagca aatgaagtac cgggaccgtg
     2641 cagctgaacg cagagaaaag tatggcatcc ccgagccgcc agagcccaag aggaggaagt
     2701 acggcggcat atccacagcc tctgtagact tcgagcagcc tactcgggac gggctgggca
     2761 gtgacaacat tggcagtcgg atgctgcagg ccatgggctg gaaagagggc agcggcctgg
     2821 gccgcaagaa gcagggcatt gtaacgccta tcgaggccca aacacgggtg cggggctccg
     2881 gcctgggtgc acggggcagc tcctacgggg tcacctcaac cgagtcctac aaggagacac
     2941 tgcacaagac aatggtgacc cgcttcaacg aggcccagtg attgccaact ttcttgtaca
     3001 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3061 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3121 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3181 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     3241 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3301 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3361 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3421 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3481 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3541 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3601 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3661 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3721 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3781 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3841 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3901 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3961 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4021 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4081 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4141 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4201 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4261 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4321 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4381 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5161 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5221 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5281 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5341 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5401 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5461 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5521 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5581 cct
//
