LOCUS       sequence-162499-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mCherry tagged BUB3 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUM BUB3
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..747
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(188..206)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     456..475
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgcgccagt cctccgacag actgagtcgc ccgctggaga ccatggtgag caagggcgag
       61 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      121 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      181 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      241 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      301 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      361 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      421 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      481 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      541 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      601 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
      661 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      721 tccaccggcg gcatggacga gctgtacatg accggttcta acgagttcaa gctgaaccag
      781 ccacccgag
//
