LOCUS       sequence-162505-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cre-dependent CA-AMPK under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, 
            Wellenstein K, Kahn BB, Sabatini BL, Lowell BB
  TITLE     A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives 
            Fasting-Induced Synaptic Plasticity in AgRP Neurons.
  JOURNAL   Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025.
  PUBMED    27321921
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025."
COMMENT     Sequence Label: pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct agcaatggag tcggttgctg
      181 cagagagctc gccagctcta gagaatgaac actttcaaga gaccccggaa tcaaacaata
      241 gtgtgtatac ttccttcatg aagtctcatc gctgctatga cctaattccc acaagttcca
      301 agttggtggt atttgacact tcgctacagg taaagaaagc cttttttgcc ctggtgacca
      361 atggtgttcg tgccgcccct ttgtgggaca gtaagaagca gagttttgtg ggcatgctga
      421 ccatcaccga cttcatcaac attttgcacc gatactataa gtcagccctg gtgcagatct
      481 acgaactgga ggagcacaag atagagacgt ggagagaggt gtacctgcag gactccttta
      541 agccacttgt ctgcatctct ccaaatgcca gcttgtttga tgctgtctct tcattaattc
      601 gaaataagat ccgcaggctc ccagttatcg acccagagtc aggcaacacc ttgtacatcc
      661 ttactcacaa gcggatcctc aagttcctca agttgtttat caccgagttc cccaagccgg
      721 agttcatgtc taagtctctc caagagctgc agattggcac ctatgccaat attgccatgg
      781 tc
//
