LOCUS       sequence-162509-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cre-dependent PAK1-AID under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, 
            Wellenstein K, Kahn BB, Sabatini BL, Lowell BB
  TITLE     A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives 
            Fasting-Induced Synaptic Plasticity in AgRP Neurons.
  JOURNAL   Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025.
  PUBMED    27321921
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025."
COMMENT     Sequence Label: pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(548..565)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct agcacaatgc atacaattca
      181 tgttggtttt gatgctgtca caggggagtt tacgggaatg ccagaacagt gggctcgctt
      241 gcttcaaaca tcaaatatca caaagtcaga gcagaagaaa aacccacagg ctgttctgga
      301 tgtgttggaa ttttataact ctaagaagac ctccaatagt cagaagtaca tgagttttac
      361 agataagtca taactcgagg cccctctccc tccccccccc ctaacgttac tggccgaagc
      421 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      481 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      541 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      601 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      661 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaa
//
