LOCUS       sequence-162510-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cre-dependent PAK1-AID under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, 
            Wellenstein K, Kahn BB, Sabatini BL, Lowell BB
  TITLE     A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives 
            Fasting-Induced Synaptic Plasticity in AgRP Neurons.
  JOURNAL   Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025.
  PUBMED    27321921
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025."
COMMENT     Sequence Label: pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     150..171
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    225..258
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    309..342
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          382..585
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(405..425)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
       61 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      121 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      181 gggatcactc tcggcatgga cgagctgtac aagtaaggcg cgccataact tcgtataatg
      241 tatgctatac gaagttatta agaggtttca tattgctaat agcagctaca atccagctac
      301 cattctgcat aacttcgtat aaagtatcct atacgaagtt attccggagt cgactctaga
      361 ggatccggta cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg
      421 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa
      481 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata
      541 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg
      601 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc
      661 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt
      721 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga
      781 ccgggccagc cttgccgccc agg
//
