LOCUS       sequence-162512-        1581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with an insertion of 1 U immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m5
FEATURES             Location/Qualifiers
     source          1..1581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(264..286)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    275..291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(299..305,306..323,324..329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(482..499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(733..752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcnncngtc gntctagagg tacccaaaac acttgatact gtatgagcat acagtataat
       61 tgcttcggta ccgctctgcg ttccgtgcga tatgcgggag aggttctagc tacaccctct
      121 ataaaaaact aatggacgag ctgtatcctt ggatacggcc ttttttatgt ttttctagag
      181 caccttccga aaaaaggtgt ttttttgcgt gaattagctg tagcgatgtc tctcgcgaat
      241 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct ccctttcggg aagcgtggcg
      841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      901 ggctgtgtgc acgaaccccc ccggttcagc cccgaccgct gcgccttatc cggtaactat
      961 cgtcttgagt ccaancccgg taagacacga cttatcggcc actgggcagc agccactggg
     1021 taacaggnat tnagcanaac cgaggtatgt nggcggtgct acaaantttc ttgnaagngg
     1081 gnggcctaac tacggctacc ctanaaaaan ngtnatttgg gtatcntgcg cnnctgnctg
     1141 aaaaccaggt taccttncgg aaaaaaaant tggtannctt ctttgaatcc ggnaaaaaaa
     1201 aaccacccgc tgggtaacgg gtggtttttt tntttngcaa ncnnncaaaa ttacccccnc
     1261 aaaaaaaaag gnatcncaaa aaaaatcctt tngaattttt ttctnacngg ggntctggac
     1321 cccnccangg aanaaaaaat ccccnntnaa gggaattttg ggcctgnnaa attttcaaaa
     1381 agggaatntt cccccaaaac cctttttana tnaaaaagga aatttttaan ccnnccaaat
     1441 tttntgggaa aaanctggtt tgaangttac aaggtttaat ngngggggcc cnttncnnag
     1501 aatgggttnn tngtctccaa aanngnngaa ncccncgggg naaaancaca aagggggggt
     1561 tnacctcgcc ccnnngttna a
//
