LOCUS       sequence-162513-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with an insertion of 2 U's immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m6
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(253..269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(266..288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    277..293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(301..307,308..325,326..331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(484..501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(735..754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 angcgcagnn gncctngagg atcccaaaac acttgatact ggtatgagca tacagtataa
       61 ttgcttcggt accgctctgc gttccgtgcg atatgcggga gaggttctag ctacaccctc
      121 tataaaaaac taattggacg agctgtatcc ttggatacgg ccttttttat gtttttctag
      181 agcaccttcc gaaaaaaggt gtttttttgc gtgaattagc tgtagcgatg tctctcgcga
      241 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      961 tcttgagtcc aacccggtaa gaaacgaact tatcgccact ggcagcagcc actgggtaac
     1021 aggattanca nancgaggta tgtaggcggt gctacaaaat tcttgaannn ggngncctaa
     1081 ctacggctac nctaaaaaaa cagtatttgg tatctgcgcc cnngctgaaa acnagtnacc
     1141 ttcnggaaaa aaantnggna anctnntgaa tcngncaaaa naaacnaccn nctggnancg
     1201 ggngggtttt tttnttttgc aagccnccaa aatacncccc aaaaaaaaag gaatcccaan
     1261 aaaaatcntt tgaatttttt nccncggggg tntgaacccn ccaggggaan caaaaacccc
     1321 cgttaagggg atttgggtcc agnaaatttc aaaaaggaat nncccccaaa atccntttaa
     1381 attaaaaaan aanttttaaa ccccccaagt ttanttgaaa naantggggt cgannantnc
     1441 caanggttaa cnggggggnc cttttccngn aanggtnntt tnnttancna aagggcngaa
     1501 nnccccnggg naaaaaanaa aanngggggn ctcccttgnn c
//
