LOCUS       sequence-162515-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with two G to U mutations immediately following the 
            riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m8
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(264..286)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    275..291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(299..305,306..323,324..329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(482..499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(733..752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggcgcagtc gnctctagag ggtcccaaaa cacttgatac tgtatgagca tacagtataa
       61 ttgcttcggt accgctctgc gttccgtgcg atatgcggga gaggttctag ctacaccctc
      121 tataaaaaac taattacgag ctgtatcctt ggatacggcc ttttttatgt ttttctagag
      181 caccttccga aaaaaggtgt ttttttgcgt gaattagctg tagcgatgtc tctcgcgaat
      241 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      841 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt a
//
