LOCUS       sequence-162516-        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GRB7, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GRB7_WT_V5
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     919..935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     919..935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     940..959
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        940..958
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    976..1075
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2473..2484
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2672..2771)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2787..2804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2787..2803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2796..2818)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2867..2953
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3045..3072
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3231..3248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3402..3990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3482..3501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4083..4386,1..488))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
       61 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
      121 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
      181 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
      241 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
      301 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
      361 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
      421 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      481 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
      541 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
      601 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
      661 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
      721 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
      781 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
      841 gtctagagga ggcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
      901 gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc actatagggg
      961 atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     1021 caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagt tgggcagagg
     1081 gccacccgcg tgcagcaatg gcgcagcaag cacggcagga tgccgcggtt cagctggtgg
     1141 aactccacga gctgcagcag gtcagtgaag cgggtctggc catcatccat gctgaagtac
     1201 aggcggccct cctcctcgct cggcaggatg agataatgct tcactttctg caggtggcac
     1261 aaagagagga caaagccctg ggggttccgc tgactctccc ggaccaggaa caggccgtct
     1321 accaagccct gctgtccaat aagccgctgg ctctcctcac gggaaatgcg cccgtggaac
     1381 cagagttggg tgcggtggat ggctgcactg aggctcgtgc cggaggctgg catgggcagg
     1441 ctgaggcggt ggtttgtctt cttcctccag gcctgggcct cctccagggc cacactcaga
     1501 gcctcccggg ggttctcaat gacacgccca gcatggccag agaagtccat ggccaccagg
     1561 gtattatctg aggcacttct caagggtggg gagcccaaac aagatggatg cagatggcga
     1621 gactgtgcct gctggtaatt cttgtacagc tgcaccccgt acttgaagag gcggaaggca
     1681 gccagccagc aggtgcggct ctgctcatct tcactgcaga agatccgaag ccccttgtgg
     1741 ccatttcgaa gcttgttggg cttgacacag aaaccgaagt cagtgggcat cccgtagagc
     1801 ttgcggccct gcgtcaccac gtacacgttg gactcgttca catctgccac gtactgcagg
     1861 tgcctcggat ccttagaggt gcccttggtg gagtaataga ggccagatcg gcgcaagaag
     1921 cagaaaaagc gtttccaaag cttccgtcct gaaccccgca gctgcagaaa gccctggatc
     1981 tcaggaaagc tgccagcatt caggaagttc tggatgaggt cttcatggga tataccagtg
     2041 tgtgcatcga gacagctgga gaccattttt tctgggaaca gggagtgtgg ggagctcttg
     2101 aacagttcgt acttggcgaa gtttttccgg aagacgaagc ggctatctcc gcccacgggc
     2161 caggcagcct gcacttccac cacggactcg tggtcctcca aaccccgctc cagtgctagg
     2221 tgggggtggc actccaccag cccccaggtc tcgtcgctca aggcgtgagc tcgctgcacc
     2281 agcatttcac acacgtggcg agctgtggca cctgctgcca cctccacaga cctgcaggcc
     2341 ccatcctcac tgtacacctt tactacatgg gggcggctgg catcgcgggg gagcagcccc
     2401 cttgcactgg aggggccccc gagaattggg ctctgtgagg gaggactgca gagctcaggg
     2461 aaggggttgg ggatagaggg gagggaggtg gcacgcctct cctcctctcg aagtttcctg
     2521 ccggtggttg ggatgaggag aggctgggac ctctttacct cctcaggcag aggggtatca
     2581 gggggccggg gagtcccagg aggggtccca ggggctgggc aaaggtcttc cggagagctg
     2641 ctaagatgag gtggagacag atccagctcc atgccaactt ttttgtacaa agttggcatt
     2701 ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2761 atcattattt ggggcccgag cttaagactg gccgtcgttt tacaacgtcg tgactgggaa
     2821 aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     2881 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     2941 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg
     3001 gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac
     3061 ggatggcctt tttgcgtttc tacaaactct tcctggctag cggtacgcgt attaattgcg
     3121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4021 gacgctcagt ggaacgacgc gtaactcacg ttaagggatt ttggtcatgg gtggctcgag
     4081 ggttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     4141 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     4201 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     4261 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     4321 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     4381 gcctca
//
