LOCUS       sequence-162517-        3660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIH1D1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PIH1D1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     263..279
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     263..279
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     284..303
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        284..302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    320..419
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1290..1389)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1405..1422)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1405..1421)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1414..1436)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1485..1571
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1663..1690
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1849..1866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2020..2608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2100..2119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2701..3492)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
       61 gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt
      121 ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg
      181 gctggtctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
      241 ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata
      301 ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt
      361 gcaacaaatt gataagcaat gctttcttat aatgccaact ttgtacaaga aagttgggca
      421 agaaggcacc agcagaagcg gcatggccac cattaattgc tttctcttcc ggtggaaggc
      481 tgccttgctc tcatgagagt tgatctgcag cgggatataa gcgtctagat gatacagctg
      541 ctgggggccc cccatcacca ggcggttctc cccgatctcc agcgacagcc ccagggctcc
      601 atccagtttg gggaggtcaa tttcggccaa gaggaggtcg ggggcttcca gccacaggtt
      661 caggtgaggc ttttcagggc ctgactcagc tctcccgggg gcgggcgtgt acaggtcccc
      721 cagctcctgg atccgaggac gctgctccga gcggatgttc tgctgcgaga tggagcccat
      781 gaatggccgg ttcttcatca tgcgccattc cggattcagc tgcaagttgt atttgtcctc
      841 aaggccctcc ctggcgatgg tgatcacgag ctcccgcaag aaatcgctgt tctgcatcct
      901 ccggtagaag tcgctgttga cagctacgtc gtaggcggta catccctggc cttttgcatc
      961 cagttctgca tgaggctctc ccagactcat ggggatgcga aacccagctt ggtcctcctc
     1021 tagcatctga agcagctcct cctcggtcac gtcggcggga ggagggatag agggggagtg
     1081 gcagatgttg atgaaaacct tcccttccga ggagttggtc tttatgcaga aaccaggctg
     1141 aggctggatt tgtgtcgatt ctggtctggt tgtctgggct tgctggagct ccttcgaggc
     1201 ctgcagcagc agctcctcaa atcgcgccga atcagcaccg atcgcctccg cctcgcttag
     1261 ctccagtccc agcagcttcg ggttcgccat gccaactttt ttgtacaaag ttggcattat
     1321 aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1381 cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
     1441 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     1501 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1561 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc
     1621 gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg
     1681 atggcctttt tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt
     1741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2461 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2641 cgctcagtgg aacgacgcgt aactcacgtt aagggatttt ggtcatgggt ggctcgaggg
     2701 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     2761 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     2821 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     2881 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     2941 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     3001 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     3061 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     3121 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     3181 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     3241 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     3301 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     3361 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     3421 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     3481 cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3541 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3601 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
//
