LOCUS       sequence-162521-        3622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CBR1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CBR1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(620..719)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(735..752)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(735..751)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..766)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      815..901
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      993..1020
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1179..1196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1350..1938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1430..1449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2031..2822)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3253..3269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3253..3269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3274..3293
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3274..3292
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3310..3409
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atcctggaca gaacggtgac gccaatcttc gtcaccccgt atgcgctgct gggccagccc
       61 tccttctggt gcactccctt ctttgtatcc tccacaaact tgttcatgag ccccaccagc
      121 tcctcctcag tgatggtctc actgcggaac ttctgctgca gctctgggct gcagctttta
      181 agggctctga cgctcatgat gctagatacg ttcaccactc tcccttgggg ttttattaga
      241 gggagtaatt ctgtgcacac atctcgggta ccaaagaaat ttgttttcat cgtcacttca
      301 gcttgaatat gaaagggtgt gggatcagca accttgaagg cgatgcccgc gttgttgacc
      361 agcacgtcca ggcccccgta ctccttgcgc aggaagtcgc gcagggcgcg gatgctctgc
      421 agatcgtcga tgtccagctg gtggaagcgc gggctcaggc cctccgcctg cagctgctgt
      481 acggccgcct ggccccgcgt cacgtcccgc gccgtgagca ccacgtcccc cgagaacagc
      541 cggcacaggt cgcgcacgat ggccaagccg atgcccttgt tgcctccagt caccagcgct
      601 acatggatgc cggacgacat gccaactttt ttgtacaaag ttggcattat aaaaaagcat
      661 tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg
      721 ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccatgct
      781 agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      901 tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg
      961 cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt
     1021 tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg
     1081 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1141 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1201 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1261 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1321 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1381 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1441 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1501 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1561 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1621 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1681 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1741 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1801 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1861 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1921 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1981 aacgacgcgt aactcacgtt aagggatttt ggtcatgggt ggctcgaggg ttatttgccg
     2041 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
     2101 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     2161 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     2221 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     2281 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     2341 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
     2401 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
     2461 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
     2521 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
     2581 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
     2641 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
     2701 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
     2761 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
     2821 ataatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
     2881 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2941 tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     3001 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc
     3061 ttacgaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg
     3121 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag
     3181 aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
     3241 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca
     3301 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     3361 gataagcaat gctttcttat aatgccaact ttgtacaaga aagttgggtg accactgttc
     3421 aactctcttc tctgaaacaa attgtccatg gggaccctca gcatctgggg gcaaaagggc
     3481 caagtacaca ggggtctctg caccttcttc tgggctcttg gtggccttgg gtcccgccat
     3541 gtcagttctc acccaccctg ggcagcaggc attcaggagg atcttgtccc ctttcctctg
     3601 ctcactcagt ttcctggcgt gg
//
