LOCUS       sequence-162526-        6192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TERT, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_TERT_p.L593F
FEATURES             Location/Qualifiers
     source          1..6192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             192..3590
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHFKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     protein_bind    complement(3592..3691)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3708..3727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3709..3727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3732..3748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3732..3748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4179..4970
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5063..5651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5552..5571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5805..5822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5981..6008)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6100..6186)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccgcgc gctccccgct gccgagccgt gcgctccctg ctgcgcagcc
      241 actaccgcga ggtgctgccg ctggccacgt tcgtgcggcg cctggggccc cagggctggc
      301 ggctggtgca gcgcggggac ccggcggctt tccgcgcgct ggtggcccag tgcctggtgt
      361 gcgtgccctg ggacgcacgg ccgccccccg ccgccccctc cttccgccag gtgtcctgcc
      421 tgaaggagct ggtggcccga gtgctgcaga ggctgtgcga gcgcggcgcg aagaacgtgc
      481 tggccttcgg cttcgcgctg ctggacgggg cccgcggggg cccccccgag gccttcacca
      541 ccagcgtgcg cagctacctg cccaacacgg tgaccgacgc actgcggggg agcggggcgt
      601 gggggctgct gctgcgccgc gtgggcgacg acgtgctggt tcacctgctg gcacgctgcg
      661 cgctctttgt gctggtggct cccagctgcg cctaccaggt gtgcgggccg ccgctgtacc
      721 agctcggcgc tgccactcag gcccggcccc cgccacacgc tagtggaccc cgaaggcgtc
      781 tgggatgcga acgggcctgg aaccatagcg tcagggaggc cggggtcccc ctgggcctgc
      841 cagccccggg tgcgaggagg cgcgggggca gtgccagccg aagtctgccg ttgcccaaga
      901 ggcccaggcg tggcgctgcc cctgagccgg agcggacgcc cgttgggcag gggtcctggg
      961 cccacccggg caggacgcgt ggaccgagtg accgtggttt ctgtgtggtg tcacctgcca
     1021 gacccgccga agaagccacc tctttggagg gtgcgctctc tggcacgcgc cactcccacc
     1081 catccgtggg ccgccagcac cacgcgggcc ccccatccac atcgcggcca ccacgtccct
     1141 gggacacgcc ttgtcccccg gtgtacgccg agaccaagca cttcctctac tcctcaggcg
     1201 acaaggagca gctgcggccc tccttcctac tcagctctct gaggcccagc ctgactggcg
     1261 ctcggaggct cgtggagacc atctttctgg gttccaggcc ctggatgcca gggactcccc
     1321 gcaggttgcc ccgcctgccc cagcgctact ggcaaatgcg gcccctgttt ctggagctgc
     1381 ttgggaacca cgcgcagtgc ccctacgggg tgctcctcaa gacgcactgc ccgctgcgag
     1441 ctgcggtcac cccagcagcc ggtgtctgtg cccgggagaa gccccagggc tctgtggcgg
     1501 cccccgagga ggaggacaca gacccccgtc gcctggtgca gctgctccgc cagcacagca
     1561 gcccctggca ggtgtacggc ttcgtgcggg cctgcctgcg ccggctggtg cccccaggcc
     1621 tctggggctc caggcacaac gaacgccgct tcctcaggaa caccaagaag ttcatctccc
     1681 tggggaagca tgccaagctc tcgctgcagg agctgacgtg gaagatgagc gtgcggggct
     1741 gcgcttggct gcgcaggagc ccaggggttg gctgtgttcc ggccgcagag caccgtctgc
     1801 gtgaggagat cctggccaag ttcctgcact ggctgatgag tgtgtacgtc gtcgagctgc
     1861 tcaggtcttt cttttatgtc acggagacca cgtttcaaaa gaacaggctc tttttctacc
     1921 ggaagagtgt ctggagcaag ttgcaaagca ttggaatcag acagcacttc aagagggtgc
     1981 agctgcggga gctgtcggaa gcagaggtca ggcagcatcg ggaagccagg cccgccctgc
     2041 tgacgtccag actccgcttc atccccaagc ctgacgggct gcggccgatt gtgaacatgg
     2101 actacgtcgt gggagccaga acgttccgca gagaaaagag ggccgagcgt ctcacctcga
     2161 gggtgaaggc actgttcagc gtgctcaact acgagcgggc gcggcgcccc ggcctcctgg
     2221 gcgcctctgt gctgggcctg gacgatatcc acagggcctg gcgcaccttc gtgctgcgtg
     2281 tgcgggccca ggacccgccg cctgagctgt actttgtcaa ggtggatgtg acgggcgcgt
     2341 acgacaccat cccccaggac aggctcacgg aggtcatcgc cagcatcatc aaaccccaga
     2401 acacgtactg cgtgcgtcgg tatgccgtgg tccagaaggc cgcccatggg cacgtccgca
     2461 aggccttcaa gagccacgtc tctaccttga cagacctcca gccgtacatg cgacagttcg
     2521 tggctcacct gcaggagacc agcccgctga gggatgccgt cgtcatcgag cagagctcct
     2581 ccctgaatga ggccagcagt ggcctcttcg acgtcttcct acgcttcatg tgccaccacg
     2641 ccgtgcgcat caggggcaag tcctacgtcc agtgccaggg gatcccgcag ggctccatcc
     2701 tctccacgct gctctgcagc ctgtgctacg gcgacatgga gaacaagctg tttgcgggga
     2761 ttcggcggga cgggctgctc ctgcgtttgg tggatgattt cttgttggtg acacctcacc
     2821 tcacccacgc gaaaaccttc ctcaggaccc tggtccgagg tgtccctgag tatggctgcg
     2881 tggtgaactt gcggaagaca gtggtgaact tccctgtaga agacgaggcc ctgggtggca
     2941 cggcttttgt tcagatgccg gcccacggcc tattcccctg gtgcggcctg ctgctggata
     3001 cccggaccct ggaggtgcag agcgactact ccagctatgc ccggacctcc atcagagcca
     3061 gtctcacctt caaccgcggc ttcaaggctg ggaggaacat gcgtcgcaaa ctctttgggg
     3121 tcttgcggct gaagtgtcac agcctgtttc tggatttgca ggtgaacagc ctccagacgg
     3181 tgtgcaccaa catctacaag atcctcctgc tgcaggcgta caggtttcac gcatgtgtgc
     3241 tgcagctccc atttcatcag caagtttgga agaaccccac atttttcctg cgcgtcatct
     3301 ctgacacggc ctccctctgc tactccatcc tgaaagccaa gaacgcaggg atgtcgctgg
     3361 gggccaaggg cgccgccggc cctctgccct ccgaggccgt gcagtggctg tgccaccaag
     3421 cattcctgct caagctgact cgacaccgtg tcacctacgt gccactcctg gggtcactca
     3481 ggacagccca gacgcagctg agtcggaagc tcccggggac gacgctgact gccctggagg
     3541 ccgcagccaa cccggcactg ccctcagact tcaagaccat cctggactga ttgccaactt
     3601 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     3661 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     3721 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     3781 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     3841 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     3901 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     3961 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     4021 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     4081 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     4141 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     4201 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     4261 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     4321 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     4381 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     4441 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     4501 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     4561 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     4621 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     4681 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     4741 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     4801 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     4861 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     4921 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     4981 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     5041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5401 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5461 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5521 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5581 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5641 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5701 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5761 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5821 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5881 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5941 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     6001 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     6061 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     6121 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     6181 tatttgatgc ct
//
