LOCUS       sequence-162532-        4887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZBTB24, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ZBTB24_p.G693S
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2287..2386)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2403..2422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2404..2422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2427..2443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2427..2443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2874..3665
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3758..4346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4247..4266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4500..4517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4676..4703)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4795..4881)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcagaa acatcgccag agccttctgg gcagcttgtt gtacactcag
      241 acgctcacag tgacactgtg ctggccagtt ttgaggatca gaggaagaaa ggcttcctct
      301 gtgacattac tttaatcgtg gagaatgtac atttccgggc ccacaaagcc ttacttgctg
      361 ccagtagtga atacttctca atgatgtttg cagaagaggg ggaaatcggc caatccattt
      421 atatgctgga aggcatggtt gcagacacct ttggtatcct gctggaattt atctacacag
      481 gttatctcca tgccagtgag aaaagtacag aacaaatcct ggctactgct cagttcttaa
      541 aagtctatga cctggtaaag gcttacacag acttccaaaa taatcatagc tccccaaagc
      601 caacaacttt gaacactgct ggtgccccag tggttgttat ctctaataag aaaaacgatc
      661 ctccaaagcg gaaacgggga agaccaaaaa aagtcaatac attgcaggag gagaaatcag
      721 aactggctgc agaggaagaa atacagttaa gagtgaacaa ttcagttcag aatagacaaa
      781 actttgtggt taaaggagac agtggtgtac tgaatgagca aattgcagca aaagaaaagg
      841 aagaatcgga gcctacttgt gagccaagta gagaggagga aatgccagtt gaaaaagatg
      901 agaactatga tcccaagacc gaggatggcc aggcaagcca gagtcgatac agcaagcgga
      961 ggatttggag atccgtcaaa cttaaagatt acaaacttgt tggggatcaa gaggaccatg
     1021 gttcagccaa gaggatctgt ggaaggagaa agcgccctgg aggccctgag gcccgctgta
     1081 aagactgtgg caaggtcttt aagtacaatc actttttagc aatccaccag aggagccaca
     1141 caggggagcg acctttcaaa tgtaatgagt gtggaaaagg ctttgcccag aagcactcgc
     1201 tacaggtcca caccaggatg cacacaggcg agcggccgta cacctgcacc gtgtgcagca
     1261 aggctctgac caccaagcac tcactgctgg agcacatgag cctgcactca ggacagaagt
     1321 cttttacctg tgatcaatgc ggaaaatatt tcagccagaa cagacagcta aagagccatt
     1381 accgagttca tacaggccac tcattaccgg aatgcaaaga ctgccatcgc aaattcatgg
     1441 atgtgtctca gctaaagaaa catctgcgaa cacacacagg tgagaagcca tttacttgtg
     1501 aaatctgtgg caaatctttc acagcaaaga gttctcttca gacccacatc agaatccatc
     1561 gaggagaaaa gccatactcc tgtggcattt gtggcaaatc cttctctgac tccagtgcca
     1621 aaaggagaca ctgcattcta cacactggca agaagccttt ctcctgccct gagtgtaact
     1681 tacagtttgc tcgcttagac aacttgaagg ctcacttgaa aattcatagc aaggagaagc
     1741 atgcttcaga tgccagcagc atttctggca gtagtaatac agaagaggtc aggaatattc
     1801 ttcagctaca gccatatcaa ctctctacct cgggagagca ggaaattcag cttctcgtaa
     1861 ccgattctgt acataacatc aatttcatgc ccggtcctag ccagggaatc agcattgtga
     1921 ctgcagagag ttcccaaaac atgactgcag accaggctgc taatcttacc ctgctcacgc
     1981 agcagccaga gcaactgcag aatttaattc tttcagctca acaggagcaa acagaacaca
     2041 ttcagagcct caatatgatt gaaagccaga tggggccctc acaaacagag ccagtgcacg
     2101 tgatcactct gtccaaggaa acactggaac atcttcatgc ccatcaagag caaacagagg
     2161 agctccattt agctacaagt acttcagatc cagctcagca cctgcagctg acacaggagc
     2221 ctggtccgcc accacccact caccacgtgc cccagccaac gccacttagc caggagcaga
     2281 gctgattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2341 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2401 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2461 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2521 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2581 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2641 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2701 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2761 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2821 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2881 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2941 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     3001 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     3061 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3121 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     3181 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3241 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3301 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3361 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3421 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3481 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3541 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3601 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3661 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4381 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4441 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4501 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4561 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4621 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4681 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4741 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4801 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4861 ctgagccttt cgttttattt gatgcct
//
